COMPARACIÓN DE UNA PRUEBA DE ELISA ESTÁNDAR Y ELISA DE RANGO EXTENDIDO PARA LA ENFERMEDAD INFECCIOSA DE LA BURSA EN AVES

• Autores: Salas M., Magali, Icochea D’A., Eliana, Gavidia Ch., César
• Formato: artículo
• Fecha de Publicación: 2002
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/1708
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1708
• Nivel de acceso: acceso abierto
• Materia: Virus de la Enfermedad Infecciosa de la Bursa (VEIB); anticuerpos; ELISA Estándar (ELISA IBD); ELISA de Rango Extendido (ELISA IBD-XR); Infectious Bursal Disease virus (IBDV); antibodies; ELISA Standard (ELISA IBD); ELISA of Extended Range (ELISA IBD-XR)

A total of 180 paired serum samples from 3 breeder groups (broiler’s grandparents and parents), and layers) were used to detect antibodies against the Infectious Bursal Disease virus through the ELISA test. Animals were tested at three ages (1-3 days, 16, and 27 weeks of age). Two commercial kits were tested: the standard ELISA (ELISA-IBD) and the new named Extended Range ELISA (ELISA-XR). The latter detected higher titres of antibodies than the ELISA-IBD. Broiler’s parents had the higher levels of antibodies indicating better immune response than the other two groups. The titre distribution had a more uniform distribution using the ELISA-IBD rather that the ELISA-XR; however all titres were above the minimum accepted ranges when the ELISA-XR was used (p<0.05). The results indicated that the new ELISA-XR was more efficient in detecting antibodies against the Infectious Bursal Disease virus.