Effect of Ley-Leygo and Triladyl extenders and two equilibration times on the freezing of boar semen

• Autores: Reyes-González, Erika Abigail, Centurión-Castro, Fernando, Uc-Espinosa, Nayeli, Ek-Mex, Jesús Enrique, Peralta-Torres, Jorge Alonso, Segura-Correa, José Candelario
• Formato: artículo
• Fecha de Publicación: 2022
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/22758
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/22758
• Nivel de acceso: acceso abierto
• Materia: criopreservación; semen congelado; reproducción; eyaculado; cryopreservation; frozen semen; reproduction; ejaculate

The aim of this study was to evaluate the effect of Ley-Leygo and Triladyl® extenders and two equilibrium times on individual motility (IM), plasma membrane integrity (PMI), mitochondrial activity (MA) and intact acrosome (IA) in frozen-thawed boar semen. A rich fraction of a total of 20 ejaculates from five boars of a commercial line was used. All ejaculates had a minimum of 80% motility and 85% normal spermatozoa. Each ejaculate was divided into four aliquots: Ley-Leygo and Triladyl® extenders and two equilibrium times (0 and 1 h). The semen samples were thawed at 37 °C for 20 s. The results of the IM and the PMI did not have significant effects for the factors evaluated (p>0.05). There was a significant difference for MA (p<0.05) between the Ley-Leygo (43.57%) and Triladyl® (51.7%) diluents. The IA was 46% for Ley-Leygo and 25% for Triladyl® (p<0.05). The percentage of IA with the equilibration time of 1 h was greater than that of 0 h (p<0.05). It is concluded that the Triladyl® diluent improved the MA and the equilibration time of 1 h improved the percentage of IA.