Identification of genes related to drought in native potatoes using RNA-Seq

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The recent advent RNA sequencing technology (RNA-Seq), a massively parallel sequencing method for transcriptome analysis, provides an opportunity to understand the expression profile of plants in response to biotic and abiotic stress. In this study, the mRNA was sequencing from leaves and roots of t...

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Detalles Bibliográficos
Autores: Torres, Yerisf, Lozano, Roberto, Merino, Carlos, Orjeda, Gisella
Formato: artículo
Fecha de Publicación:2013
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/5208
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/5208
Nivel de acceso:acceso abierto
Materia:RNA-Seq
transcriptome
drought
potato
mRNA.
transcriptoma
sequía
papa
mRNA. Abstract
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spelling Identification of genes related to drought in native potatoes using RNA-SeqIdentificación de genes relacionados a sequía en papas nativas empleando RNA-SeqTorres, YerisfLozano, RobertoMerino, CarlosOrjeda, GisellaRNA-SeqtranscriptomedroughtpotatomRNA.RNA-SeqtranscriptomasequíapapamRNA. AbstractThe recent advent RNA sequencing technology (RNA-Seq), a massively parallel sequencing method for transcriptome analysis, provides an opportunity to understand the expression profile of plants in response to biotic and abiotic stress. In this study, the mRNA was sequencing from leaves and roots of two native potato varieties at different levels of drought. Fifty-base-pair reads from whole mRNAs were mapped to the potato genomic sequence: 75 – 82% mapped uniquely to the genome, 6 – 14% mapped to several locations in the genome and 9 – 12% had no match in the genome. Comparing expression profiles, 887 to 1925 genes were found to be induced/repressed by drought in the sensible variety and 998 to 1995 in the tolerant. This research provides valuable information for future studies and deeper understanding of the molecular mechanism of drought resistance in potato and related species.El reciente desarrollo del RNA-Seq, un método de secuenciamiento masivo en paralelo para el análisis de transcriptomas permite conocer el perfil de expresión de las plantas en respuesta a estrés de tipo abiótico y biótico. En este estudio, se secuenció el mRNA proveniente de hojas y raíces de dos variedades de papas nativas expuestas a diferentes niveles de sequía. Lecturas o readsde 50 pares de bases provenientes de mRNA se mapearon al genoma de papa: 75 – 82% mapearon a posiciones únicas, 6 – 14% mapearon a múltiples posiciones y 9 – 12% no mapearon a posición alguna del genoma. Comparando los perfiles de expresión, se encontraron entre 887 – 1925 genes inducidos/reprimidos por sequía en la variedad sensible y 998 – 1995 en la tolerante. Este estudio generó información de gran valor que podrá ser utilizada en futuros estudios para comprender mejor los mecanismos moleculares de resistencia a sequía en papa y especies cercanas.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2013-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/520810.15381/rpb.v20i3.5208Revista Peruana de Biología; Vol. 20 Núm. 3 (2013); 211 - 214Revista Peruana de Biología; Vol. 20 No. 3 (2013); 211 - 2141727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/5208/4380Derechos de autor 2013 Yerisf Torres, Roberto Lozano, Carlos Merino, Gisella Orjedahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/52082020-05-20T19:43:10Z
dc.title.none.fl_str_mv Identification of genes related to drought in native potatoes using RNA-Seq
Identificación de genes relacionados a sequía en papas nativas empleando RNA-Seq
title Identification of genes related to drought in native potatoes using RNA-Seq
spellingShingle Identification of genes related to drought in native potatoes using RNA-Seq
Torres, Yerisf
RNA-Seq
transcriptome
drought
potato
mRNA.
RNA-Seq
transcriptoma
sequía
papa
mRNA. Abstract
title_short Identification of genes related to drought in native potatoes using RNA-Seq
title_full Identification of genes related to drought in native potatoes using RNA-Seq
title_fullStr Identification of genes related to drought in native potatoes using RNA-Seq
title_full_unstemmed Identification of genes related to drought in native potatoes using RNA-Seq
title_sort Identification of genes related to drought in native potatoes using RNA-Seq
dc.creator.none.fl_str_mv Torres, Yerisf
Lozano, Roberto
Merino, Carlos
Orjeda, Gisella
author Torres, Yerisf
author_facet Torres, Yerisf
Lozano, Roberto
Merino, Carlos
Orjeda, Gisella
author_role author
author2 Lozano, Roberto
Merino, Carlos
Orjeda, Gisella
author2_role author
author
author
dc.subject.none.fl_str_mv RNA-Seq
transcriptome
drought
potato
mRNA.
RNA-Seq
transcriptoma
sequía
papa
mRNA. Abstract
topic RNA-Seq
transcriptome
drought
potato
mRNA.
RNA-Seq
transcriptoma
sequía
papa
mRNA. Abstract
description The recent advent RNA sequencing technology (RNA-Seq), a massively parallel sequencing method for transcriptome analysis, provides an opportunity to understand the expression profile of plants in response to biotic and abiotic stress. In this study, the mRNA was sequencing from leaves and roots of two native potato varieties at different levels of drought. Fifty-base-pair reads from whole mRNAs were mapped to the potato genomic sequence: 75 – 82% mapped uniquely to the genome, 6 – 14% mapped to several locations in the genome and 9 – 12% had no match in the genome. Comparing expression profiles, 887 to 1925 genes were found to be induced/repressed by drought in the sensible variety and 998 to 1995 in the tolerant. This research provides valuable information for future studies and deeper understanding of the molecular mechanism of drought resistance in potato and related species.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/5208
10.15381/rpb.v20i3.5208
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/5208
identifier_str_mv 10.15381/rpb.v20i3.5208
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/5208/4380
dc.rights.none.fl_str_mv Derechos de autor 2013 Yerisf Torres, Roberto Lozano, Carlos Merino, Gisella Orjeda
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2013 Yerisf Torres, Roberto Lozano, Carlos Merino, Gisella Orjeda
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 20 Núm. 3 (2013); 211 - 214
Revista Peruana de Biología; Vol. 20 No. 3 (2013); 211 - 214
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238309959565312
score 13.914502
Identification of genes related to drought in native potatoes using RNA-Seq
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