Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico
Descripción del Articulo
A cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for...
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2020 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/17336 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336 |
| Nivel de acceso: | acceso abierto |
| Materia: | dogs indirect immunofluorescence Leishmania spp PCR trypanosomatids Trypanosoma cruzi inmunofluorescencia indirecta perros tripanosomátidos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico Frecuencia serológica y molecular de Leishmania spp y Trypanosoma cruzi y factores asociados en perros con signos sugestivos de infección por tripanosomátidos en Quintana Roo, México |
| title |
Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico |
| spellingShingle |
Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico Salazar Grosskelwing, Enrique dogs indirect immunofluorescence Leishmania spp PCR trypanosomatids Trypanosoma cruzi inmunofluorescencia indirecta Leishmania spp PCR perros tripanosomátidos Trypanosoma cruzi |
| title_short |
Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico |
| title_full |
Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico |
| title_fullStr |
Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico |
| title_full_unstemmed |
Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico |
| title_sort |
Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, Mexico |
| dc.creator.none.fl_str_mv |
Salazar Grosskelwing, Enrique Bolio González, Manuel Emilio Rodríguez Vivas, Roger Iván Miró Corrales, Guadalupe Escobedo Ortegón, Francisco Javier Ruíz Piña, Hugo Antonio Loría Cervera, Elsy Nalleli |
| author |
Salazar Grosskelwing, Enrique |
| author_facet |
Salazar Grosskelwing, Enrique Bolio González, Manuel Emilio Rodríguez Vivas, Roger Iván Miró Corrales, Guadalupe Escobedo Ortegón, Francisco Javier Ruíz Piña, Hugo Antonio Loría Cervera, Elsy Nalleli |
| author_role |
author |
| author2 |
Bolio González, Manuel Emilio Rodríguez Vivas, Roger Iván Miró Corrales, Guadalupe Escobedo Ortegón, Francisco Javier Ruíz Piña, Hugo Antonio Loría Cervera, Elsy Nalleli |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
dogs indirect immunofluorescence Leishmania spp PCR trypanosomatids Trypanosoma cruzi inmunofluorescencia indirecta Leishmania spp PCR perros tripanosomátidos Trypanosoma cruzi |
| topic |
dogs indirect immunofluorescence Leishmania spp PCR trypanosomatids Trypanosoma cruzi inmunofluorescencia indirecta Leishmania spp PCR perros tripanosomátidos Trypanosoma cruzi |
| description |
A cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for detection of anti-Leishmania infantum and immunochromatic test for the detection of anti-Trypanosoma cruzi antibodies in serum were performed. In addition, molecular analyses were performed using PCR to detect Leishmania spp in bone marrow and skin and T. cruzi in bone marrow. Histopathological analyses of the skin and cytology of the bone marrow were carried out in search of lesions and trypanosomatid agents. The results showed that 3 (7.5%) and 6 (15%) dogs with anti-L. infantum and T. cruzi antibodies were detected, respectively. Molecular analyses detected 17 dogs positive for infection by T. cruzi (42.5%) and none for infection by Leishmania spp. Dogs with low body condition showed greater seropositivity to T. cruzi using Fisher’s exact test (p<0.05). Parasites of the genus Leishmania did not actively participate in the pathological processes of the animals; however, a high frequency of animals infected with T. cruzi was observed. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-11-24 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336 10.15381/rivep.v31i4.17336 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336 |
| identifier_str_mv |
10.15381/rivep.v31i4.17336 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336/16048 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e17336 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e17336 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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1795238232521179136 |
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Serological and molecular frequency of Leishmania spp and Trypanosoma cruzi and associated factors in dogs with signs suggestive of trypanosomatid infection in Quintana Roo, MexicoFrecuencia serológica y molecular de Leishmania spp y Trypanosoma cruzi y factores asociados en perros con signos sugestivos de infección por tripanosomátidos en Quintana Roo, MéxicoSalazar Grosskelwing, EnriqueBolio González, Manuel EmilioRodríguez Vivas, Roger IvánMiró Corrales, GuadalupeEscobedo Ortegón, Francisco JavierRuíz Piña, Hugo AntonioLoría Cervera, Elsy Nallelidogsindirect immunofluorescenceLeishmania sppPCRtrypanosomatidsTrypanosoma cruziinmunofluorescencia indirectaLeishmania sppPCRperrostripanosomátidosTrypanosoma cruziA cross-sectional study was conducted with a sample of 40 dogs with clinical signs suggestive of infection by Leishmania spp and Trypanosoma cruzi in the municipality of Lázaro Cárdenas, Quintana Roo, Mexico. Blood, skin and bone marrow samples were taken. Indirect immunofluorescence (IFI) tests for detection of anti-Leishmania infantum and immunochromatic test for the detection of anti-Trypanosoma cruzi antibodies in serum were performed. In addition, molecular analyses were performed using PCR to detect Leishmania spp in bone marrow and skin and T. cruzi in bone marrow. Histopathological analyses of the skin and cytology of the bone marrow were carried out in search of lesions and trypanosomatid agents. The results showed that 3 (7.5%) and 6 (15%) dogs with anti-L. infantum and T. cruzi antibodies were detected, respectively. Molecular analyses detected 17 dogs positive for infection by T. cruzi (42.5%) and none for infection by Leishmania spp. Dogs with low body condition showed greater seropositivity to T. cruzi using Fisher’s exact test (p<0.05). Parasites of the genus Leishmania did not actively participate in the pathological processes of the animals; however, a high frequency of animals infected with T. cruzi was observed.Se realizó un estudio transversal con una muestra de 40 perros con signos clínicos sugestivos de infección por Leishmania spp y Trypanosoma cruzi en el municipio de Lázaro Cárdenas, Quintana Roo, México. Se tomaron muestras de sangre, piel y médula ósea. Se realizaron las pruebas de inmunofluorescencia indirecta (IFI) para detección de anticuerpos anti-Leishmania infantum e inmunocromática para detección de anticuerpos anti-Trypanosoma cruzi en suero. Además, se realizaron análisis moleculares, mediante PCR para la detección de Leishmania spp en médula ósea y piel y T. cruzi en médula ósea. Se realizaron análisis histopatológicos de piel y citológicos de médula ósea en busca de lesiones y agentes tripanosomátidos. Se detectaron 3 (7.5%) y 6 (15%) perros con anticuerpos anti-L. infantum y T. cruzi, respectivamente. En los análisis moleculares se detectaron 17 perros positivos a la infección por T. cruzi (42.5%) y ninguno a la infección por Leishmania spp. Los perros con baja condición corporal presentaron mayor seropositividad a T. cruzi mediante la prueba exacta de Fisher (p<0.05). Los parásitos del género Leishmania no participaron activamente en los procesos patológicos de los animales estudiados; sin embargo, se observa una alta frecuencia de animales infectados por T. cruzi.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-11-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1733610.15381/rivep.v31i4.17336Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e17336Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e173361682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17336/16048Derechos de autor 2020 Enrique Salazar Grosskelwing, Manuel Emilio Bolio González, Roger Iván Rodríguez Vivas, Guadalupe Miró Corrales, Francisco Javier Escobedo Ortegón, Hugo Antonio Ruíz Piña, Elsy Nalleli Loría Cerverahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/173362020-11-26T23:08:49Z |
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13.947759 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
