Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)

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In this work, the poison of Phymactis papillosa collected in Ancón bay has been studied biochemically. The venom was obtained by hypotonic shock and then lyophilized. Electrophoretic analysis of the soluble poison showed the presence of 5 protein bands with molecular weights between 5 and 25.1 kDa.T...

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Detalles Bibliográficos
Autores: Cuya, Antony, Escobar, Enrique
Formato: artículo
Fecha de Publicación:2017
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/13901
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/13901
Nivel de acceso:acceso abierto
Materia:veneno
anémona de mar
toxinas
neurotoxinas
poison
sea anemone
toxins
neurotoxins
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spelling Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)Estudio bioquímico del veneno de la anémona de mar Phymactis papillosa (Actiniidae)Cuya, AntonyEscobar, Enriquevenenoanémona de martoxinasneurotoxinaspoisonsea anemonetoxinsneurotoxinsIn this work, the poison of Phymactis papillosa collected in Ancón bay has been studied biochemically. The venom was obtained by hypotonic shock and then lyophilized. Electrophoretic analysis of the soluble poison showed the presence of 5 protein bands with molecular weights between 5 and 25.1 kDa.The soluble venom was fractionated by filtration chromatography on a Sephadex G-50 column, yielding four protein peaks (I, II, III and IV). In the soluble venom and collected fractions was measured protease activity, phospholipase, hyaluronidase, acid phosphatase and alkaline phosphatase; as well as hemolytic and neurotoxic activity. Proteolytic activity on casein was found in the soluble venom and peaks I and III. Was not detected phospholipase activity, hyaluronidase, acid phosphatase and alkaline phosphatase. Hemolytic activity on human red cells tested, was found in the soluble venom and peak II. Finally, the soluble venom as the peak III showed be neurotoxic when injected into white mice intraperitoneally. It is concluded that the soluble venom of P. papillosa has proteolytic, hemolytic and neurotoxic activity.En este trabajo se ha estudiado bioquímicamente el veneno de Phymactis papillosa, colectadas en la bahía de Ancón. El veneno fue obtenido mediante shock hipotónico y luego se liofilizó. El análisis electroforético del veneno soluble mostró la presencia de 5 bandas proteicas con pesos moleculares entre 5 y 25.1 kDa.El veneno soluble fue fraccionado por cromatografía de filtración en una columna de Sephadex G-50, obteniéndose cuatro picos de proteína (I, II, III y IV). Tanto en el veneno soluble como en las fracciones colectadas se midió actividad de proteasa, fosfolipasa, hialuronidasa, fosfatasa ácida y fosfatasa alcalina; así como, actividad hemolítica y neurotóxica. Se encontró actividad proteolítica sobre caseína, en el veneno soluble y en los picos I y III. No se detectó actividad de fosfolipasa, hialuronidasa, fosfatasa ácida y fosfatasa alcalina. La actividad hemolítica, ensayada sobre eritrocitos humanos, se encontró en el veneno soluble y en el pico II. Finalmente, tanto el veneno soluble como el pico III mostraron ser neurotóxicos al ser inyectados en ratones albinos vía intraperitoneal. Se concluye que el veneno soluble de P. papillosa tiene actividad proteolítica, hemolítica y neurotóxica.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2017-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1390110.15381/rpb.v24i3.13901Revista Peruana de Biología; Vol. 24 Núm. 3 (2017); 303-310Revista Peruana de Biología; Vol. 24 No. 3 (2017); 303-3101727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/13901/12279Derechos de autor 2017 Antony Cuya, Enrique Escobarhttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/139012018-06-23T13:32:30Z
dc.title.none.fl_str_mv Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
Estudio bioquímico del veneno de la anémona de mar Phymactis papillosa (Actiniidae)
title Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
spellingShingle Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
Cuya, Antony
veneno
anémona de mar
toxinas
neurotoxinas
poison
sea anemone
toxins
neurotoxins
title_short Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
title_full Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
title_fullStr Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
title_full_unstemmed Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
title_sort Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
dc.creator.none.fl_str_mv Cuya, Antony
Escobar, Enrique
author Cuya, Antony
author_facet Cuya, Antony
Escobar, Enrique
author_role author
author2 Escobar, Enrique
author2_role author
dc.subject.none.fl_str_mv veneno
anémona de mar
toxinas
neurotoxinas
poison
sea anemone
toxins
neurotoxins
topic veneno
anémona de mar
toxinas
neurotoxinas
poison
sea anemone
toxins
neurotoxins
description In this work, the poison of Phymactis papillosa collected in Ancón bay has been studied biochemically. The venom was obtained by hypotonic shock and then lyophilized. Electrophoretic analysis of the soluble poison showed the presence of 5 protein bands with molecular weights between 5 and 25.1 kDa.The soluble venom was fractionated by filtration chromatography on a Sephadex G-50 column, yielding four protein peaks (I, II, III and IV). In the soluble venom and collected fractions was measured protease activity, phospholipase, hyaluronidase, acid phosphatase and alkaline phosphatase; as well as hemolytic and neurotoxic activity. Proteolytic activity on casein was found in the soluble venom and peaks I and III. Was not detected phospholipase activity, hyaluronidase, acid phosphatase and alkaline phosphatase. Hemolytic activity on human red cells tested, was found in the soluble venom and peak II. Finally, the soluble venom as the peak III showed be neurotoxic when injected into white mice intraperitoneally. It is concluded that the soluble venom of P. papillosa has proteolytic, hemolytic and neurotoxic activity.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/13901
10.15381/rpb.v24i3.13901
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/13901
identifier_str_mv 10.15381/rpb.v24i3.13901
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/13901/12279
dc.rights.none.fl_str_mv Derechos de autor 2017 Antony Cuya, Enrique Escobar
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2017 Antony Cuya, Enrique Escobar
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 24 Núm. 3 (2017); 303-310
Revista Peruana de Biología; Vol. 24 No. 3 (2017); 303-310
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238312002191360
score 13.948833
Biochemical study of venom sea anemone Phymactis papillosa (Actiniidae)
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