Application of Bacillus Subtilis to modify the properties of concrete

• Autor: Huayanca-Hernández, Máximo Edilberto
• Formato: artículo
• Fecha de Publicación: 2026
• Institución: Universidad Nacional Federico Villarreal
• Repositorio: Revistas - Universidad Nacional Federico Villarreal
• Lenguaje: español
• OAI Identifier: oai:ojs2.revistas.unfv.edu.pe:article/2032
• Enlace del recurso: https://revistas.unfv.edu.pe/RCV/article/view/2032
• Nivel de acceso: acceso abierto
• Materia: Hormigón; biotecnología; materiales de construcción; ingenieria civil; tecnología de materiales; Concrete; biotechnology; construction materials; civil engineering; materials technology

This study evaluates the effect of adding unencapsulated Bacillus subtilis (BS) bacteria in various concentrations (0, 187, and 312 ml/m3) on the physical, mechanical, and self-repair properties of concrete. It is an applied, quantitative study with a quasi-experimental design. National Bacillus Subtilis (BSN) and Imported Bacillus Subtilis (BSI) were used, and the concrete was designed for a strength of 210 kg/cm². The results showed that incorporating BS significantly modifies the behavior of concrete. The dosage of 187 ml/m3 (milliliters per cubic meter of concrete) of BSI increased settlement by 66.67% compared to the control mixture. compressive strength at 28 days improved by 25.60% with BSI and 14.42% with BSN, attributable to calcium carbonate precipitation induced by bacterial activity. Considering the self-repair of the mixture with 312 ml/m3 of BSN, whose initial average width and length were 0.45 mm and 6.47 cm, respectively, there was a reduction in length of 1.32 cm on average in five of sixty specimens produced. at 365 days, demonstrating the partial viability of using non-encapsulated BS to produce self-repairing concrete. The use of BS in a proportion of 187 ml/m3 is recommended to improve workability and mechanical strength, and concentrations higher than 312 ml/m3 should be explored to further investigate self-repairing capacity.