LIVER HISTOPATHOLOGY OF ECHINOCOCCUS GRANULOSUS INFECTION IN SUS SCROFA FROM BRAZIL

• Autores: Almeida B, Fernanda, Oliveira S, Magda, Neves H, Renata, Santos A, Jessica, Oliveira V, Albanita, Machado- Silva, José Roberto, Rodrigues-Silva, Rosângela
• Formato: artículo
• Fecha de Publicación: 2011
• Institución: Universidad Nacional Federico Villarreal
• Repositorio: Revistas - Universidad Nacional Federico Villarreal
• Lenguaje: español
• OAI Identifier: oai:ojs2.revistas.unfv.edu.pe:article/1032
• Enlace del recurso: https://revistas.unfv.edu.pe/NH/article/view/1032
• Nivel de acceso: acceso abierto
• Materia: Brazil; Echinococcus granulosus; histopathology; liver; Sus scrofa.; Brasil; hígado; histopatología

Museum specimens represent a valuable resource for helminthological research. We examined formalin-fixed livers preserved for periods in excess of 50 years from naturally infected pigs in archival material from the Helminthological Collection of the Oswaldo Cruz Institute (CHIOC), Oswaldo Cruz Foundation (FIOCRUZ). Microscopic examination revealed evidence of parasites in unilocular cystic structures containing a bladder worm vesicle lined with a thin laminated membrane. The inner germinal layer contained parasite debris and a proteinaceous hydatid-like fluid. The hepatic parenchyma showed a dense inflammatory infiltrate near the cyst, thick-walled arterial vessels, and occasionally necrosis. The adjacent hepatic parenchyma showed inter- and intra-lobular thin fibrosis, inflammatory cell infiltration and occasionally necrosis. Other histo-pathological sections showed a thick laminated wall cystic structure with brood capsules in the germinal layer containing small round projections with central internal vesicles on the inner surface, suggesting the presence of protoscolices. Given that Echinococcus multilocularis does not occur in Brazil, our histological findings were identified as Echinococcus granulosus in origin. In conclusion, the protocol presented in this study highlights the possibility of achieving remarkably high quality histological results in archival formalin-fixed and paraffin-embedded tissues retrieved from museum specimens that have been preserved for many years.