Pre-basic seed potato (Solanum tuberosum L.) production using temporary immersion bioreactors

• Autores: Tapia, M.de L., Arbizu, C., Beraún, F., J., Lorenzo, Escalona, M.
• Formato: artículo
• Fecha de Publicación: 2018
• Institución: Universidad Nacional Agraria La Molina
• Repositorio: Revistas - Universidad Nacional Agraria La Molina
• Lenguaje: inglés
• OAI Identifier: oai:revistas.lamolina.edu.pe:article/1127
• Enlace del recurso: https://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1127
• Nivel de acceso: acceso abierto
• Materia: Microtubers; temporary immersion bioreactor; pre-basic seed; microtuberization.

Potato is an important food and cash crop in Perú. Its production is limited due to seed quality, and other biotic and abiotic factors. Here we explore a new alternative for potato pre-basic seed production named temporary immersion bioreactor (TIB). Potato microtubers production using TIB comprises two phases: multiplication and microtuberization. During the multiplication phase, we used 50 segments of four nodes from a semi-solid medium with an immersion and frequency time of five minutes and three hours, respectively. In addition, a photoperiod of 16 hours of light and eight hours of darkness during 28 days were employed. For the microtuberization phase and under darkness conditions during 60 days, we used 80 g/l of sucrose and 30 ml/explant as a volume for the medium. Then, it was possible to obtain five, six, and 2.5 microtubers per explant of potato varieties “Canchan INIA”, “Capiro”, and “Papa 3”, respectively.