Primary structure of a PLA2 , deduced from mRNA extracted from whole venom of Crotalus durissus terrificus

• Autores: Ronald Navarro Oviedo, Bertha Roxana Mestas Valdivia, Patricia Woll Toso
• Formato: artículo
• Fecha de Publicación: 2017
• Institución: Universidad Católica de Santa María
• Repositorio: Revistas - Universidad Católica de Santa María
• Lenguaje: español
• OAI Identifier: oai:ojs.revistas.ucsm.edu.pe:article/3
• Enlace del recurso: https://revistas.ucsm.edu.pe/ojs/index.php/veritas/article/view/3
• Nivel de acceso: acceso abierto
• Materia: PLA2; cDNA; Crotalus; durissus; terrificus

From the whole venom of Crotalus durissus terrificus from, Tambopata-Madre de Dios, mRNA encoding a fosfolipase A2 (PLA2) protein has been isolated, using a simple technique. That is, cDNA encoding the PLA2 D49, has been amplified by RT-PCR.Analysis of cDNA encoding PLA2, has shown that this open reading frame of 414 bp encoding a polypeptide of 138 amino acids with a signal peptide of 16 amino acid residues, followed by a polypeptide sequence of 122 amino acids corresponding to PLA2. The analysis also shows that the protein is composed of 33 charged amino acids: 11 amino acids acidic, 20 basic amino acids, 44 polar amino acids and 28 hydrophobic amino acids, having an isoelectric point of 8.58 and a molecular mass of 14312.52 Da. The homology and phylogenetic tree analysis shows that this PLA2 has a high degree of homology and belongs to the basic PLA2 N6F24D49.