TOTAL SELENIUM AND CELULAR VIABILITY FOR OBTAINING SELENIUM-YEAST

• Autores: Rodríguez Best, Carmen Alicia, Ceroni Galloso, Mario, Villegas Silva, Elvito Fabián, Rebaza Cárdenas, Teresa Dámaris
• Formato: artículo
• Fecha de Publicación: 2019
• Institución: Sociedad Química del Perú
• Repositorio: Revista de la Sociedad Química del Perú
• Lenguaje: español
• OAI Identifier: oai:rsqp.revistas.sqperu.org.pe:article/264
• Enlace del recurso: http://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/264
• Nivel de acceso: acceso abierto
• Materia: total selenium; selenium-yeast; selenium; cellular viability; selenio total; selenio-levadura; selenito; viabilidad celular

Selenium-yeast is obtained by the culture of Saccharomyces cerevisiae LALVIN Bourgovin RC 212 in the medium YEPD, with sodium selenite addition (concentrations of 2 to 10 mg Se(IV)/L) during exponential growth phase, after 48 hours at 30°C and 200 rpm. Total intracellular selenium quantification in the selenium-yeast is feasible with the starch-iodine spectrophotometric method at 590 nm and the addition of 8 mg Se(IV)/L yields the most biomass, a >90% cellular viability, pink coloration and a total intracellular selenium of 738±30 mg/kg in dry basis Finally, the used methodology to find the cellular viability and the analytical method proposed to determine the total intracellular selenium are accessible for monitoring the bioprocess.