Diagnostic performance of the rapid fasciorap test based on the immunochromatography or lateral flow technique for the detection of antibodies in patients with human fasciolosis

• Autores: Davelois Atac, Kelly Roxana, Jara Campos, Cesar Augusto, Coronado Figarella, Andrea Victoria, Escalante Gavidia, Omar Julio, Escalante Añorga, Hermes Mario
• Formato: artículo
• Fecha de Publicación: 2022
• Institución: Sociedad de Gastroenterología del Perú
• Repositorio: Revista de Gastroenterología del Perú
• Lenguaje: español
• OAI Identifier: oai:ojs.revistagastroperu.com:article/1374
• Enlace del recurso: http://www.revistagastroperu.com/index.php/rgp/article/view/1374
• Nivel de acceso: acceso abierto
• Materia: Fasciola hepatica; Antigens; Gold colloid; Sensitivity and specificity; Antígenos; Oro coloidal; Sensibilidad

Objective: To determine the diagnostic performance of the immunochromatography technique or lateral flow for the detection of antibodies in patients with human fasciolosis. Materials and methods: Observational, prospective and cross-sectional study. We have developed a lateral flow test (Fasciorap) for the serological diagnosis of fasciolosis due to F. hepatica, composed of excretion-secretion antigens of adult forms conjugated with orocolloid of 40 nm and a protein A and IgG of rabbit anti F. hepatica as detector reagents in the test and control line, flanked by pads in a cassette. 240 sera were evaluated, 120 positive, 50 sera from patients with other parasites, 20 from patients with infectious diseases and 50 sera from non-parasitized people, the interpretation of results was performed by visual inspection 15 minutes after applying the samples. Results: The test detected the presence of antibodies in the serum of patients with fasciolosis, reaching a sensitivity of 92.5%, a specificity of 94.17%, a positive predictive value of 94.07% and a negative predictive value of 92.62%; with 100% agreement on repeatability and reproducibility. Conclusions: Fasciorap detects cases of fascioliasis, therefore, it is a potential diagnostic test in endemic areas where point-of-care testing is required.