Protein directed evolution
Descripción del Articulo
Directed evolution allows us to explore protein functionalities not required in the natural environment. It mimics natural genetic processes and selective pressures. This approach is used when the molecular basis is not completely understood and rational design is a difficult task. This approach con...
| Autor: | |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2008 |
| Institución: | Pontificia Universidad Católica del Perú |
| Repositorio: | Revistas - Pontificia Universidad Católica del Perú |
| Lenguaje: | español |
| OAI Identifier: | oai:revistaspuc:article/2592 |
| Enlace del recurso: | http://revistas.pucp.edu.pe/index.php/quimica/article/view/2592 |
| Nivel de acceso: | acceso abierto |
| Materia: | Directed evolution Water in oil emulsions Taq polymerase Lac repressor PCR Error prone PCR Compartmentalized self replication (CSR) Artificially Expanded Genetic Information System (AEGIS) |
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Protein directed evolutionLaos, RobertoDirected evolutionWater in oil emulsionsTaq polymeraseLac repressorPCRError prone PCRCompartmentalized self replication (CSR)Artificially Expanded Genetic Information System (AEGIS)Directed evolution allows us to explore protein functionalities not required in the natural environment. It mimics natural genetic processes and selective pressures. This approach is used when the molecular basis is not completely understood and rational design is a difficult task. This approach consists of serial cycles of consecutive diversification and selection which eventually lead to the accumulation of beneficial mutations. Here are presented two cases where directed evolution is used to modify two different proteins: Taq polymerase, enzyme used for DNA extension in PCR, and the LacI repressor protein which regulates gene expression on E.coli.Evolución dirigida de proteínas: La evolución dirigida es una técnica que nos permite explorar funciones enzimáticas que no son requeridas en el ambiente natural. Esta técnica, simula procesos genéticos naturales y de selección. Esta estrategia se utiliza cuando un diseño racional es muy complicado. Consiste en una repetición de ciclos de diversificación y selección que llevan a la acumulación de mutaciones benéficas. Aquí se presenta dos ejemplos de evolución dirigida con los cuales se ha trabajado directamente: la ADN polimerasa del organismo Thermus aquaticus usada comúnmente en PCR, y la proteína LacI que regula la expresión de genes usados para el metabolismo de lactosa en E. Coli.Pontificia Universidad Católica del Perú2008-05-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistas.pucp.edu.pe/index.php/quimica/article/view/2592Revista de Química; Vol. 22 Núm. 1-2 (2008); 33-462518-28031012-3946reponame:Revistas - Pontificia Universidad Católica del Perúinstname:Pontificia Universidad Católica del Perúinstacron:PUCPspahttp://revistas.pucp.edu.pe/index.php/quimica/article/view/2592/2540Derechos de autor 2016 Revista de Químicahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:revistaspuc:article/25922025-07-04T15:13:46Z |
| dc.title.none.fl_str_mv |
Protein directed evolution |
| title |
Protein directed evolution |
| spellingShingle |
Protein directed evolution Laos, Roberto Directed evolution Water in oil emulsions Taq polymerase Lac repressor PCR Error prone PCR Compartmentalized self replication (CSR) Artificially Expanded Genetic Information System (AEGIS) |
| title_short |
Protein directed evolution |
| title_full |
Protein directed evolution |
| title_fullStr |
Protein directed evolution |
| title_full_unstemmed |
Protein directed evolution |
| title_sort |
Protein directed evolution |
| dc.creator.none.fl_str_mv |
Laos, Roberto |
| author |
Laos, Roberto |
| author_facet |
Laos, Roberto |
| author_role |
author |
| dc.subject.none.fl_str_mv |
Directed evolution Water in oil emulsions Taq polymerase Lac repressor PCR Error prone PCR Compartmentalized self replication (CSR) Artificially Expanded Genetic Information System (AEGIS) |
| topic |
Directed evolution Water in oil emulsions Taq polymerase Lac repressor PCR Error prone PCR Compartmentalized self replication (CSR) Artificially Expanded Genetic Information System (AEGIS) |
| description |
Directed evolution allows us to explore protein functionalities not required in the natural environment. It mimics natural genetic processes and selective pressures. This approach is used when the molecular basis is not completely understood and rational design is a difficult task. This approach consists of serial cycles of consecutive diversification and selection which eventually lead to the accumulation of beneficial mutations. Here are presented two cases where directed evolution is used to modify two different proteins: Taq polymerase, enzyme used for DNA extension in PCR, and the LacI repressor protein which regulates gene expression on E.coli. |
| publishDate |
2008 |
| dc.date.none.fl_str_mv |
2008-05-29 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://revistas.pucp.edu.pe/index.php/quimica/article/view/2592 |
| url |
http://revistas.pucp.edu.pe/index.php/quimica/article/view/2592 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
http://revistas.pucp.edu.pe/index.php/quimica/article/view/2592/2540 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2016 Revista de Química http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2016 Revista de Química http://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Pontificia Universidad Católica del Perú |
| publisher.none.fl_str_mv |
Pontificia Universidad Católica del Perú |
| dc.source.none.fl_str_mv |
Revista de Química; Vol. 22 Núm. 1-2 (2008); 33-46 2518-2803 1012-3946 reponame:Revistas - Pontificia Universidad Católica del Perú instname:Pontificia Universidad Católica del Perú instacron:PUCP |
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Pontificia Universidad Católica del Perú |
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PUCP |
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PUCP |
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Revistas - Pontificia Universidad Católica del Perú |
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Revistas - Pontificia Universidad Católica del Perú |
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1836736780528779264 |
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13.924177 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
