Clonality of multidrogoresistants Klebsiella pneumoniae circulating at the National Maternal and Perinatal Institute of Lima, Peru (2015-2018)

• Autores: Bieli-Bianchi, Gabriela Soza, Acuña Fernández, Hortencia, Pons, María Jesús
• Formato: artículo
• Fecha de Publicación: 2021
• Institución: Instituto Nacional Materno Perinatal
• Repositorio: Revista Peruana de Investigación Materno Perinatal
• Lenguaje: español
• OAI Identifier: oai:investigacionmaternoperinatal.inmp.gob.pe:article/223
• Enlace del recurso: https://investigacionmaternoperinatal.inmp.gob.pe/index.php/rpinmp/article/view/223
• Nivel de acceso: acceso abierto
• Materia: Resistencia antibacteriana; clonalidad; Klebsiella pneumoniae; Antibiotic resistance; clonality

Objective: To determine the clonality relationship in Klebsiella pneumoniae strains that present multiresistance circulating in the Maternal-Perinatal Institute of Lima, Peru. Methodology: the present study is retrospective, experimental, transversal and includes K.pneumoniae strains isolated during the period 2015-2018. Antimicrobial sensitivity levels were analyzed using the diffusion technique interpreted according to the CLSI 2019. The clonal relationship was established by the technique of Pulsed Field Electrophoresis Gel (PFGE). Results: A total of 36 K. pneumoniae isolates from different clinical origins were analyzed, 23 (64%) blood cultures, 9 (25%) secretions and 4 (11%) stool samples. High levels of antimicrobial resistance were found in cotrimoxazole (97%), cephalosporin (67%-89%) and gentamicin (67%). It should be noted that decreased sensitivity is important in quinolones (36-47%), and moderate to Piperacillin-tazobactam, also in nitrofurantoin and chloramphenicol (25%), while Amikacin presented only 14% resistance. A 78% of the strains were extended spectrum beta-lactamase (ESBL) producing strains. PFGE showed 16 different pulses, showing related strains in the same space and time.    Conclusion: The levels of resistance found were high, and the number of related strains was also important, showing a transmission of K.pneumoniae between different patients, showing a dissemination of the microorganism.