Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)

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Background: Peru is endemic to the human T-lymphotropic virus type 1 (HTLV-1), for these reasons it is important to know the reliability of the diagnostic tests used in the country, in order to continue their use or not. The objective was to evaluate the performance of three serological tests ELISA...

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Detalles Bibliográficos
Autores: Miranda-Ulloa, Eduardo, Romero-Ruiz, Soledad, Valverde-Ticlia, Fanny, Briceño-Espinoza, Ronal, Acuña, Maribel
Formato: artículo
Fecha de Publicación:2023
Institución:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
Repositorio:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
Lenguaje:español
OAI Identifier:oai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/1516
Enlace del recurso:https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1516
Nivel de acceso:acceso abierto
Materia:HTLV-1
ELISA
Técnica del anticuerpo fluorescente indirecta
Fluorescent antibody technique indirect
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spelling Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)Evaluación de tres pruebas serológicas para la detección de anticuerpos contra el virus linfotrópico T humano tipo 1 (HTLV-1)Miranda-Ulloa, EduardoRomero-Ruiz, SoledadValverde-Ticlia, Fanny Briceño-Espinoza, Ronal Acuña, MaribelHTLV-1ELISATécnica del anticuerpo fluorescente indirecta HTLV-1ELISAFluorescent antibody technique indirect Background: Peru is endemic to the human T-lymphotropic virus type 1 (HTLV-1), for these reasons it is important to know the reliability of the diagnostic tests used in the country, in order to continue their use or not. The objective was to evaluate the performance of three serological tests ELISA Murex, ELISA Wantai and IFI INS-Peru for the detection of anti-HTLV-1 antibodies against Peruvian samples. The study. The three tests were evaluated against 382 sera: 215 positive and 167 negative for HTLV-1 (Gold Standard: immunoblot). Findings. IFI had no false positives, Wantai had more false negatives (seven) and Murex more false positives (eight). The three tests showed results above 95% for the estimated parameters of diagnostic accuracy. Conclusions. IIF INS-Perú and ELISA Murex had good diagnostic performance for the detection of antibodies against HTLV-1 and are good candidates to continue being used in Peru.Introducción: El Perú es endémico al virus linfotrópico T humano tipo 1 (HTLV-1), por esas razones es importante conocer la fiabilidad de las pruebas diagnósticas que se usan en el país, con la finalidad de continuar o no su uso. El objetivo fue evaluar el rendimiento de tres pruebas serológicas ELISA Murex, ELISA Wantai e IFI INS-Perú para la detección de anticuerpos anti HTLV-1 frente a muestras peruanas. El estudio. Las tres pruebas fueron evaluadas frente a 382 sueros: 215 positivos y 167 negativos a HTLV-1 (Gold Standar: inmunoblot). Hallazgos. IFI no presentó falsos positivos, Wantai tuvo más falsos negativos (siete) y Murex más falsos positivos (ocho). Las tres pruebas mostraron resultados superiores a 95% para los parámetros estimados de exactitud diagnóstica. Conclusiones. IFI INS-Perú y ELISA Murex tuvieron buen rendimiento diagnóstico para la detección de anticuerpos contra HTLV-1 y son buenos candidatos para continuar siendo usados en Perú.Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo2023-06-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/151610.35434/rcmhnaaa.2023.161.1516Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 16 No. 1 (2023): Rev. Cuerpo Med. HNAAA, January - March; e1516Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 16 Núm. 1 (2023): Rev. Cuerpo Med. HNAAA, Enero - Marzo; e15162227-47312225-510910.35434/rcmhnaaa.2023.161reponame:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjoinstname:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjoinstacron:HNAAAspahttps://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1516/762Derechos de autor 2023 Eduardo Miranda-Ulloa, Soledad Romero-Ruiz, Fanny Valverde-Ticlia, Ronal Briceño-Espinoza, Maribel Acuñahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/15162025-03-11T13:38:08Z
dc.title.none.fl_str_mv Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
Evaluación de tres pruebas serológicas para la detección de anticuerpos contra el virus linfotrópico T humano tipo 1 (HTLV-1)
title Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
spellingShingle Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
Miranda-Ulloa, Eduardo
HTLV-1
ELISA
Técnica del anticuerpo fluorescente indirecta
HTLV-1
ELISA
Fluorescent antibody technique indirect
title_short Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
title_full Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
title_fullStr Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
title_full_unstemmed Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
title_sort Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
dc.creator.none.fl_str_mv Miranda-Ulloa, Eduardo
Romero-Ruiz, Soledad
Valverde-Ticlia, Fanny
Briceño-Espinoza, Ronal
Acuña, Maribel
author Miranda-Ulloa, Eduardo
author_facet Miranda-Ulloa, Eduardo
Romero-Ruiz, Soledad
Valverde-Ticlia, Fanny
Briceño-Espinoza, Ronal
Acuña, Maribel
author_role author
author2 Romero-Ruiz, Soledad
Valverde-Ticlia, Fanny
Briceño-Espinoza, Ronal
Acuña, Maribel
author2_role author
author
author
author
dc.subject.none.fl_str_mv HTLV-1
ELISA
Técnica del anticuerpo fluorescente indirecta
HTLV-1
ELISA
Fluorescent antibody technique indirect
topic HTLV-1
ELISA
Técnica del anticuerpo fluorescente indirecta
HTLV-1
ELISA
Fluorescent antibody technique indirect
description Background: Peru is endemic to the human T-lymphotropic virus type 1 (HTLV-1), for these reasons it is important to know the reliability of the diagnostic tests used in the country, in order to continue their use or not. The objective was to evaluate the performance of three serological tests ELISA Murex, ELISA Wantai and IFI INS-Peru for the detection of anti-HTLV-1 antibodies against Peruvian samples. The study. The three tests were evaluated against 382 sera: 215 positive and 167 negative for HTLV-1 (Gold Standard: immunoblot). Findings. IFI had no false positives, Wantai had more false negatives (seven) and Murex more false positives (eight). The three tests showed results above 95% for the estimated parameters of diagnostic accuracy. Conclusions. IIF INS-Perú and ELISA Murex had good diagnostic performance for the detection of antibodies against HTLV-1 and are good candidates to continue being used in Peru.
publishDate 2023
dc.date.none.fl_str_mv 2023-06-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1516
10.35434/rcmhnaaa.2023.161.1516
url https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1516
identifier_str_mv 10.35434/rcmhnaaa.2023.161.1516
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1516/762
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo
publisher.none.fl_str_mv Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo
dc.source.none.fl_str_mv Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 16 No. 1 (2023): Rev. Cuerpo Med. HNAAA, January - March; e1516
Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 16 Núm. 1 (2023): Rev. Cuerpo Med. HNAAA, Enero - Marzo; e1516
2227-4731
2225-5109
10.35434/rcmhnaaa.2023.161
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instacron:HNAAA
instname_str Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
instacron_str HNAAA
institution HNAAA
reponame_str Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
collection Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
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Evaluation of three serological tests for the detection of antibodies against human T-lymphotropic virus type 1 (HTLV-1)
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