Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2
Descripción del Articulo
Objective: To optimize the internal quality control of real-time RT-PCR for the qualitative detection of SARS-CoV-2, using the Cq values of negative and positive controls. Material and method: Prospective-longitudinal study. The sample consisted of 143 Cq values for the negative aliquot and extr...
| Autor: | |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2023 |
| Institución: | Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo |
| Repositorio: | Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo |
| Lenguaje: | español |
| OAI Identifier: | oai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/1657 |
| Enlace del recurso: | https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1657 |
| Nivel de acceso: | acceso abierto |
| Materia: | Optimización Control interno de calidad RT-PCR en tiempo real SARS-CoV-2 Optimization Internal quality control Real-time RT-PCR |
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Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2Optimización del control interno de calidad de RT-PCR en tiempo real para detección cualitativa de SARS-CoV-2Torres-Gamarra, GiancarloOptimizaciónControl interno de calidadRT-PCR en tiempo realSARS-CoV-2OptimizationInternal quality controlReal-time RT-PCRSARS-CoV-2Objective: To optimize the internal quality control of real-time RT-PCR for the qualitative detection of SARS-CoV-2, using the Cq values of negative and positive controls. Material and method: Prospective-longitudinal study. The sample consisted of 143 Cq values for the negative aliquot and extraction controls, as well as for the positive control. The normal distribution of Cq values was analyzed using the Anderson-Darling (AD) test and randomness tests were applied. Control limits were calculated from 51 Cq values, and then, using control charts, to monitor 92 Cq values obtained from November 2020 to March 2021. Lot acceptance and Cpk indices were evaluated as optimization indicators. The calculations were made with the Minitab program. Results: The batches of Cq values were accepted and Cpk indices higher than 1.33 were obtained for the three types of control. Discussion: There are no published studies that apply statistical quality control to the qualitative detection of SARS-CoV-2. Conclusions: It is possible to use the Cq values of the controls to optimize the internal quality control of real-time RT-PCR for qualitative detection of SARS-CoV-2, as if it were a quantitative technique.Objetivo: Optimizar el control interno de calidad de RT-PCR en tiempo real para detección cualitativa de SARS-CoV-2, utilizando los valores Cq de controles negativos y positivos. Material y método: Estudio prospectivo-longitudinal. La muestra estuvo constituida por 143 valores Cq para los controles negativos de alicuotado y extracción, así como para el control positivo. Se analizó la distribución normal de los valores Cq mediante la prueba de Anderson-Darling (AD) y se aplicaron pruebas de aleatoriedad. Se calculó límites de control a partir de 51 valores Cq, para luego, mediante gráficas de control, monitorizar 92 valores Cq obtenidos desde noviembre del 2020 hasta marzo del 2021. Se evaluó aceptación de lote e índices Cpk como indicadores de optimización. Los cálculos se hicieron con el programa Minitab. Resultados: Se aceptaron los lotes de valores Cq y se obtuvieron índices Cpk superiores a 1.33 para los tres tipos de control. Discusión: No existen estudios publicados que apliquen control estadístico de calidad a la detección cualitativa de SARS-CoV-2. Conclusiones: Es posible utilizar los valores Cq de los controles para optimizar el control interno de calidad de RT-PCR en tiempo real para detección cualitativa de SARS-CoV-2, como si se tratara de una técnica de tipo cuantitativo.Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo2023-06-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionText and imagesTexto e imágenesapplication/pdfhttps://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/165710.35434/rcmhnaaa.2023.161.1657Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 16 No. 1 (2023): Rev. Cuerpo Med. HNAAA, January - March; e1657Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 16 Núm. 1 (2023): Rev. Cuerpo Med. HNAAA, Enero - Marzo; e16572227-47312225-510910.35434/rcmhnaaa.2023.161reponame:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjoinstname:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjoinstacron:HNAAAspahttps://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1657/795Derechos de autor 2023 Giancarlo Torres-Gamarrahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/16572025-03-11T13:38:08Z |
| dc.title.none.fl_str_mv |
Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2 Optimización del control interno de calidad de RT-PCR en tiempo real para detección cualitativa de SARS-CoV-2 |
| title |
Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2 |
| spellingShingle |
Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2 Torres-Gamarra, Giancarlo Optimización Control interno de calidad RT-PCR en tiempo real SARS-CoV-2 Optimization Internal quality control Real-time RT-PCR SARS-CoV-2 |
| title_short |
Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2 |
| title_full |
Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2 |
| title_fullStr |
Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2 |
| title_full_unstemmed |
Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2 |
| title_sort |
Real-time RT-PCR internal quality control optimization for qualitative detection of SARS-CoV-2 |
| dc.creator.none.fl_str_mv |
Torres-Gamarra, Giancarlo |
| author |
Torres-Gamarra, Giancarlo |
| author_facet |
Torres-Gamarra, Giancarlo |
| author_role |
author |
| dc.subject.none.fl_str_mv |
Optimización Control interno de calidad RT-PCR en tiempo real SARS-CoV-2 Optimization Internal quality control Real-time RT-PCR SARS-CoV-2 |
| topic |
Optimización Control interno de calidad RT-PCR en tiempo real SARS-CoV-2 Optimization Internal quality control Real-time RT-PCR SARS-CoV-2 |
| description |
Objective: To optimize the internal quality control of real-time RT-PCR for the qualitative detection of SARS-CoV-2, using the Cq values of negative and positive controls. Material and method: Prospective-longitudinal study. The sample consisted of 143 Cq values for the negative aliquot and extraction controls, as well as for the positive control. The normal distribution of Cq values was analyzed using the Anderson-Darling (AD) test and randomness tests were applied. Control limits were calculated from 51 Cq values, and then, using control charts, to monitor 92 Cq values obtained from November 2020 to March 2021. Lot acceptance and Cpk indices were evaluated as optimization indicators. The calculations were made with the Minitab program. Results: The batches of Cq values were accepted and Cpk indices higher than 1.33 were obtained for the three types of control. Discussion: There are no published studies that apply statistical quality control to the qualitative detection of SARS-CoV-2. Conclusions: It is possible to use the Cq values of the controls to optimize the internal quality control of real-time RT-PCR for qualitative detection of SARS-CoV-2, as if it were a quantitative technique. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-06-18 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text and images Texto e imágenes |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1657 10.35434/rcmhnaaa.2023.161.1657 |
| url |
https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1657 |
| identifier_str_mv |
10.35434/rcmhnaaa.2023.161.1657 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/1657/795 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2023 Giancarlo Torres-Gamarra https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2023 Giancarlo Torres-Gamarra https://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo |
| publisher.none.fl_str_mv |
Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo |
| dc.source.none.fl_str_mv |
Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 16 No. 1 (2023): Rev. Cuerpo Med. HNAAA, January - March; e1657 Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 16 Núm. 1 (2023): Rev. Cuerpo Med. HNAAA, Enero - Marzo; e1657 2227-4731 2225-5109 10.35434/rcmhnaaa.2023.161 reponame:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo instname:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo instacron:HNAAA |
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HNAAA |
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Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
