Detection of Patotypes of Escherichia coli Strains Causing Childhood Diarrhea in Health Establishments of the Lambayeque-Peru Region, 2018

• Autores: Yacarini-Martínez, Antero Enrique, Arriaga-Deza, Emma Vanesa, Beltrán-Orbegoso, Raúl Antonio
• Formato: artículo
• Fecha de Publicación: 2020
• Institución: Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
• Repositorio: Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
• Lenguaje: español
• OAI Identifier: oai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/741
• Enlace del recurso: https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/741
• Nivel de acceso: acceso abierto
• Materia: Escherichia coli; genes; factores de virulencia; diarrea; Virulence factors; diarrhea

Objetive. To identify pathotypes of virulence genes of Escherichia coli from diarrheal samples of children under five years of age in the Lambayeque Region, in the period April-September 2018. The study: Descriptive and cross-sectional study. 75 E. coli strains from diarrheogenic samples were analyzed using real-time Multiplex PCR and primers for E. coli pathotype genes: daaD, aggR, eaeA, stx, ipaH and st. Findings: 34.66% (26/75) of virulence genes were detected from the total isolates. The enterotoxigenic E. coli pathotype presented the highest frequency, 18.67% (14/75) of the total isolates. It was also detected that the enterotoxigenic E. coli pathotype appeared in 53.86% (14/26) of the total positive strains. Also, in 11.54% of positive strains (3/26) they presented more than one pathotype. Conclusion: Molecular epidemiology will help a better, adequate and timely diagnosis of childhood diarrhea in this vulnerable population.