Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids

• Autores: Muehlig A., Jahn I.J., Heidler J., Jahn M., Weber K., Sheen P., Zimic M., Cialla-May D., Popp J.
• Formato: artículo
• Fecha de Publicación: 2019
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/2712
• Enlace del recurso: https://hdl.handle.net/20.500.12390/2712; https://doi.org/10.3390/app9122511
• Nivel de acceso: acceso abierto
• Materia: Surface-enhanced Raman spectroscopy (SERS); Drug resistant mycobacteria; Lab-on-a-Chip (LoC); Personal medicine; Quantitative SERS detection of PZA and POA; http://purl.org/pe-repo/ocde/ford#3.02.18

The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 ?m for PZA and 21 ?m for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 ?m to 50 ?m for PZA and from 50 ?m to 80 ?m for POA. © 2019 by the authors.