Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria

Descripción del Articulo

366-2012
Detalles Bibliográficos
Autores: Evangelista-Vargas, D, Evangelista-Vargas, S, Valdivia, M, Santiani, A
Formato: artículo
Fecha de Publicación:2017
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/1240
Enlace del recurso:https://hdl.handle.net/20.500.12390/1240
https://doi.org/10.1111/rda.12907
Nivel de acceso:acceso abierto
Materia:Spermatozoa
Animals
Camelids, New World
Image Processing, Computer-Assisted
Male
Semen Analysis
https://purl.org/pe-repo/ocde/ford#1.06.00
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network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
title Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
spellingShingle Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
Evangelista-Vargas, D
Spermatozoa
Animals
Animals
Camelids, New World
Image Processing, Computer-Assisted
Image Processing, Computer-Assisted
Male
Semen Analysis
Semen Analysis
https://purl.org/pe-repo/ocde/ford#1.06.00
title_short Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
title_full Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
title_fullStr Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
title_full_unstemmed Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
title_sort Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
author Evangelista-Vargas, D
author_facet Evangelista-Vargas, D
Evangelista-Vargas, S
Valdivia, M
Santiani, A
author_role author
author2 Evangelista-Vargas, S
Valdivia, M
Santiani, A
author2_role author
author
author
dc.contributor.author.fl_str_mv Evangelista-Vargas, D
Evangelista-Vargas, S
Valdivia, M
Santiani, A
dc.subject.none.fl_str_mv Spermatozoa
topic Spermatozoa
Animals
Animals
Camelids, New World
Image Processing, Computer-Assisted
Image Processing, Computer-Assisted
Male
Semen Analysis
Semen Analysis
https://purl.org/pe-repo/ocde/ford#1.06.00
dc.subject.es_PE.fl_str_mv Animals
Animals
Camelids, New World
Image Processing, Computer-Assisted
Image Processing, Computer-Assisted
Male
Semen Analysis
Semen Analysis
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#1.06.00
description 366-2012
publishDate 2017
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/1240
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1111/rda.12907
dc.identifier.isi.none.fl_str_mv 395593800019
url https://hdl.handle.net/20.500.12390/1240
https://doi.org/10.1111/rda.12907
identifier_str_mv 395593800019
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Reproduction in Domestic Animals
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley and Sons Inc
publisher.none.fl_str_mv John Wiley and Sons Inc
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1844331696158146560
spelling Publicationrp03571600rp00121500rp02465500rp02362500Evangelista-Vargas, DEvangelista-Vargas, SValdivia, MSantiani, A2024-05-30T23:13:38Z2024-05-30T23:13:38Z2017https://hdl.handle.net/20.500.12390/1240https://doi.org/10.1111/rda.12907395593800019366-2012Although computer-assisted systems for sperm morphometry and morphological analysis are important tools in the study of male fertility, their use in extensive systems in alpacas is limited by factors such as the expense of equipment and the high altitudes of the Andean region. The objectives of this study were to evaluate alpaca sperm head morphometry using a nonautomated digital method and determine the frequency of sperm abnormalities based on strict criteria for sperm morphology in fertile male alpacas. Ejaculates (n = 15) from seven alpacas were collected, and sperm smears stained with modified Papanicolaou were processed. For morphometric analysis, 3,000 sperm (200 cells/sample) images were captured at 400× magnification and Quick Photo MICRO 3.0 software was used for manual measurement of basic (sperm head length, width, perimeter and area) and derived variables (ellipticity, shape factor, elongation and regularity). For morphology assessment, smears were observed at 1000× magnification according to WHO and strict criteria. Average morphometric parameters were length 5.48 μm, width 2.99 μm, perimeter 13.62 μm, area 12.43 μm2, ellipticity 1.86, shape factor 1.20, elongation 0.29 and regularity 1.05. Significant between-individual and within-individual differences were found in morphometric parameters. Based on morphometric study, sperm heads were classified as elliptical or normal (49%), long (18%), short (2%), pyriform (12%), round (9%), large (6%) and small (4%). Morphological analysis found no additional sperm head defects in 49% of normal sperm obtained by morphometry, although a 4% incidence of neck/mid-piece defects and a 16% incidence of principal-piece defects were found. We conclude that sperm head morphometry assessment in fertile alpacas using a nonautomated digital method is feasible, and that defects in sperm heads constitute the main morphological alteration (>50% of the sperm population), based on WHO and strict criteria.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengJohn Wiley and Sons IncReproduction in Domestic Animalsinfo:eu-repo/semantics/openAccessSpermatozoaAnimals-1Animals-1Camelids, New World-1Image Processing, Computer-Assisted-1Image Processing, Computer-Assisted-1Male-1Semen Analysis-1Semen Analysis-1https://purl.org/pe-repo/ocde/ford#1.06.00-1Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteriainfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/1240oai:repositorio.concytec.gob.pe:20.500.12390/12402024-05-30 16:02:01.087http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="20632789-140d-458c-a94f-3966279335db"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria</Title> <PublishedIn> <Publication> <Title>Reproduction in Domestic Animals</Title> </Publication> </PublishedIn> <PublicationDate>2017</PublicationDate> <DOI>https://doi.org/10.1111/rda.12907</DOI> <ISI-Number>395593800019</ISI-Number> <Authors> <Author> <DisplayName>Evangelista-Vargas, D</DisplayName> <Person id="rp03571" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Evangelista-Vargas, S</DisplayName> <Person id="rp00121" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Valdivia, M</DisplayName> <Person id="rp02465" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Santiani, A</DisplayName> <Person id="rp02362" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>John Wiley and Sons Inc</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Spermatozoa</Keyword> <Keyword>Animals</Keyword> <Keyword>Animals</Keyword> <Keyword>Camelids, New World</Keyword> <Keyword>Image Processing, Computer-Assisted</Keyword> <Keyword>Image Processing, Computer-Assisted</Keyword> <Keyword>Male</Keyword> <Keyword>Semen Analysis</Keyword> <Keyword>Semen Analysis</Keyword> <Abstract>Although computer-assisted systems for sperm morphometry and morphological analysis are important tools in the study of male fertility, their use in extensive systems in alpacas is limited by factors such as the expense of equipment and the high altitudes of the Andean region. The objectives of this study were to evaluate alpaca sperm head morphometry using a nonautomated digital method and determine the frequency of sperm abnormalities based on strict criteria for sperm morphology in fertile male alpacas. Ejaculates (n = 15) from seven alpacas were collected, and sperm smears stained with modified Papanicolaou were processed. For morphometric analysis, 3,000 sperm (200 cells/sample) images were captured at 400× magnification and Quick Photo MICRO 3.0 software was used for manual measurement of basic (sperm head length, width, perimeter and area) and derived variables (ellipticity, shape factor, elongation and regularity). For morphology assessment, smears were observed at 1000× magnification according to WHO and strict criteria. Average morphometric parameters were length 5.48 μm, width 2.99 μm, perimeter 13.62 μm, area 12.43 μm2, ellipticity 1.86, shape factor 1.20, elongation 0.29 and regularity 1.05. Significant between-individual and within-individual differences were found in morphometric parameters. Based on morphometric study, sperm heads were classified as elliptical or normal (49%), long (18%), short (2%), pyriform (12%), round (9%), large (6%) and small (4%). Morphological analysis found no additional sperm head defects in 49% of normal sperm obtained by morphometry, although a 4% incidence of neck/mid-piece defects and a 16% incidence of principal-piece defects were found. We conclude that sperm head morphometry assessment in fertile alpacas using a nonautomated digital method is feasible, and that defects in sperm heads constitute the main morphological alteration (&gt;50% of the sperm population), based on WHO and strict criteria.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.059308
Assessment of spermatozoa in fertile alpaca (Vicugna pacos) males: Study of sperm head morphometry using a nonautomated digital method and sperm morphology based on strict criteria
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