Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases

Descripción del Articulo

grant no. 0114-02-93-01
Detalles Bibliográficos
Autores: Córdova M., Reátegui L., Espinoza J.R.
Formato: artículo
Fecha de Publicación:1999
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/934
Enlace del recurso:https://hdl.handle.net/20.500.12390/934
https://doi.org/10.1016/S0035-9203(99)90178-5
Nivel de acceso:acceso abierto
Materia:Medicina clínica
https://purl.org/pe-repo/ocde/ford#3.02.19
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network_name_str CONCYTEC-Institucional
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spelling Publicationrp02471600rp02470600rp01901500Córdova M.Reátegui L.Espinoza J.R.2024-05-30T23:13:38Z2024-05-30T23:13:38Z1999https://hdl.handle.net/20.500.12390/934https://doi.org/10.1016/S0035-9203(99)90178-52-s2.0-0033007745grant no. 0114-02-93-01The immunodiagnosis of human infections with Fasciola hepatica using purified parasite cysteine proteinases as antigens is reported in this paper. IgG enzyme-linked immunosorbent assays (ELISAs) with 2 cysteine proteinases of 26 kDa (Fas1) and 25 kDa (Fas2), obtained from the regurgitated material of adult worms, were evaluated with serum samples from 38 patients infected with F. hepatica, from 54 patients with other parasitic infections and from 46 healthy volunteers. The diagnostic sensitivities for detection of F. hepatica infection at a serum dilution of 1 in 500 were 89% and 95% with Fas1 and Fas2 respectively. The specificity of the ELISAs was 98% for Fas1 and 100% for Fas2. IgG ELISA with Fas2 is a highly sensitive and specific assay for the immunodiagnosis of human fascioliasis.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengRoyal Society of Tropical Medicine and Hygieneinfo:eu-repo/semantics/openAccessMedicina clínicahttps://purl.org/pe-repo/ocde/ford#3.02.19-1Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinasesinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/934oai:repositorio.concytec.gob.pe:20.500.12390/9342024-05-30 15:59:57.098http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="4fdab413-b69a-456f-953d-810ffe46e037"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>1999</PublicationDate> <DOI>https://doi.org/10.1016/S0035-9203(99)90178-5</DOI> <SCP-Number>2-s2.0-0033007745</SCP-Number> <Authors> <Author> <DisplayName>Córdova M.</DisplayName> <Person id="rp02471" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Reátegui L.</DisplayName> <Person id="rp02470" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Espinoza J.R.</DisplayName> <Person id="rp01901" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Royal Society of Tropical Medicine and Hygiene</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Medicina clínica</Keyword> <Abstract>The immunodiagnosis of human infections with Fasciola hepatica using purified parasite cysteine proteinases as antigens is reported in this paper. IgG enzyme-linked immunosorbent assays (ELISAs) with 2 cysteine proteinases of 26 kDa (Fas1) and 25 kDa (Fas2), obtained from the regurgitated material of adult worms, were evaluated with serum samples from 38 patients infected with F. hepatica, from 54 patients with other parasitic infections and from 46 healthy volunteers. The diagnostic sensitivities for detection of F. hepatica infection at a serum dilution of 1 in 500 were 89% and 95% with Fas1 and Fas2 respectively. The specificity of the ELISAs was 98% for Fas1 and 100% for Fas2. IgG ELISA with Fas2 is a highly sensitive and specific assay for the immunodiagnosis of human fascioliasis.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
dc.title.none.fl_str_mv Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
title Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
spellingShingle Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
Córdova M.
Medicina clínica
https://purl.org/pe-repo/ocde/ford#3.02.19
title_short Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
title_full Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
title_fullStr Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
title_full_unstemmed Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
title_sort Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
author Córdova M.
author_facet Córdova M.
Reátegui L.
Espinoza J.R.
author_role author
author2 Reátegui L.
Espinoza J.R.
author2_role author
author
dc.contributor.author.fl_str_mv Córdova M.
Reátegui L.
Espinoza J.R.
dc.subject.none.fl_str_mv Medicina clínica
topic Medicina clínica
https://purl.org/pe-repo/ocde/ford#3.02.19
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#3.02.19
description grant no. 0114-02-93-01
publishDate 1999
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 1999
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/934
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/S0035-9203(99)90178-5
dc.identifier.scopus.none.fl_str_mv 2-s2.0-0033007745
url https://hdl.handle.net/20.500.12390/934
https://doi.org/10.1016/S0035-9203(99)90178-5
identifier_str_mv 2-s2.0-0033007745
dc.language.iso.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv Royal Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1844883098086408192
score 13.394457
Immunodiagnosis of human fascioliasis with Fasciola hepatica cysteine proteinases
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

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