The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats

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This study was supported by a grant obtained from the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the grant PROCOM 2009, No. 217-2009, Peru. G. F. participated in the design of the study and writing the paper. B. V. V. and M. G. participated in the development...

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Detalles Bibliográficos
Autores: Gonzales G.F., Vasquez V.B., Gasco M.
Formato: artículo
Fecha de Publicación:2013
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/944
Enlace del recurso:https://hdl.handle.net/20.500.12390/944
https://doi.org/10.3109/15376516.2013.802830
Nivel de acceso:acceso abierto
Materia:Planta medicinal
Botánica
https://purl.org/pe-repo/ocde/ford#1.06.10
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network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
title The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
spellingShingle The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
Gonzales G.F.
Planta medicinal
Botánica
https://purl.org/pe-repo/ocde/ford#1.06.10
title_short The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
title_full The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
title_fullStr The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
title_full_unstemmed The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
title_sort The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
author Gonzales G.F.
author_facet Gonzales G.F.
Vasquez V.B.
Gasco M.
author_role author
author2 Vasquez V.B.
Gasco M.
author2_role author
author
dc.contributor.author.fl_str_mv Gonzales G.F.
Vasquez V.B.
Gasco M.
dc.subject.none.fl_str_mv Planta medicinal
topic Planta medicinal
Botánica
https://purl.org/pe-repo/ocde/ford#1.06.10
dc.subject.es_PE.fl_str_mv Botánica
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#1.06.10
description This study was supported by a grant obtained from the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the grant PROCOM 2009, No. 217-2009, Peru. G. F. participated in the design of the study and writing the paper. B. V. V. and M. G. participated in the development of the study. All the authors revised the manuscript. Dr. Gustavo F. Gonzales is in charge of CAYENATUR EIRL, a small enterprise that develops products based on maca. No other authors have conflict of interest.
publishDate 2013
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/944
dc.identifier.doi.none.fl_str_mv https://doi.org/10.3109/15376516.2013.802830
dc.identifier.scopus.none.fl_str_mv 2-s2.0-84886397159
url https://hdl.handle.net/20.500.12390/944
https://doi.org/10.3109/15376516.2013.802830
identifier_str_mv 2-s2.0-84886397159
dc.language.iso.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor & Francis Online
publisher.none.fl_str_mv Taylor & Francis Online
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1844882993807622144
spelling Publicationrp00575500rp02583600rp00573500Gonzales G.F.Vasquez V.B.Gasco M.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2013https://hdl.handle.net/20.500.12390/944https://doi.org/10.3109/15376516.2013.8028302-s2.0-84886397159This study was supported by a grant obtained from the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the grant PROCOM 2009, No. 217-2009, Peru. G. F. participated in the design of the study and writing the paper. B. V. V. and M. G. participated in the development of the study. All the authors revised the manuscript. Dr. Gustavo F. Gonzales is in charge of CAYENATUR EIRL, a small enterprise that develops products based on maca. No other authors have conflict of interest.Transillumination technique for assessment of stages of spermatogenic cycle is a useful tool for toxicological studies. This study was designed to determine the effect of two medicinal plants on spermatogenesis in male rats using the transillumination technique. For this, the effect of the combination of a fruit with highest content of ascorbic acid (Myrciaria dubia, camu camu) and extract of black maca (Lepidium meyenii) on seminiferous tubule stages scored by transillumination on intact tubules in adult male rats was assessed. Animals were treated during seven days with vehicle, black maca, camu camu or a mixture of black maca + camu camu and assessed for daily sperm production (DSP), stages of spermatogenic cycle as well as antioxidant activity and levels of flavonoids and polyphenols. Black maca increased stages of spermiation (VII–VIII) and mitosis of germ cells (IX–XI), whereas camu camu increased stages of mitosis (IX–XI) and meiosis (XII). Mixture of maca + camu camu increased stages of spermiation, mitosis and meiosis. All treatments increased DSP (p < 0.05) and epididymal sperm count (p < 0.05). Total polyphenols, flavonoids levels and antioxidant activity were higher in camu camu (p < 0.001) than in black maca. In conclusion, M. dubia (camu camu) has potential effects improving spermatogenesis and co-administered with maca increase stages of mitosis, meiosis and spermiation of the spermatogenic cycle as assessed by the transillumination technique. This technique is becoming increasingly a useful tool for assessment spermatogenesis.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengTaylor & Francis Onlineinfo:eu-repo/semantics/openAccessPlanta medicinalBotánica-1https://purl.org/pe-repo/ocde/ford#1.06.10-1The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male ratsinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/944oai:repositorio.concytec.gob.pe:20.500.12390/9442024-05-30 16:00:03.88http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="85c33b7b-5560-45e4-ac3a-ecb1f2f99672"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2013</PublicationDate> <DOI>https://doi.org/10.3109/15376516.2013.802830</DOI> <SCP-Number>2-s2.0-84886397159</SCP-Number> <Authors> <Author> <DisplayName>Gonzales G.F.</DisplayName> <Person id="rp00575" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Vasquez V.B.</DisplayName> <Person id="rp02583" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Gasco M.</DisplayName> <Person id="rp00573" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Taylor &amp; Francis Online</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Planta medicinal</Keyword> <Keyword>Botánica</Keyword> <Abstract>Transillumination technique for assessment of stages of spermatogenic cycle is a useful tool for toxicological studies. This study was designed to determine the effect of two medicinal plants on spermatogenesis in male rats using the transillumination technique. For this, the effect of the combination of a fruit with highest content of ascorbic acid (Myrciaria dubia, camu camu) and extract of black maca (Lepidium meyenii) on seminiferous tubule stages scored by transillumination on intact tubules in adult male rats was assessed. Animals were treated during seven days with vehicle, black maca, camu camu or a mixture of black maca + camu camu and assessed for daily sperm production (DSP), stages of spermatogenic cycle as well as antioxidant activity and levels of flavonoids and polyphenols. Black maca increased stages of spermiation (VII–VIII) and mitosis of germ cells (IX–XI), whereas camu camu increased stages of mitosis (IX–XI) and meiosis (XII). Mixture of maca + camu camu increased stages of spermiation, mitosis and meiosis. All treatments increased DSP (p &lt; 0.05) and epididymal sperm count (p &lt; 0.05). Total polyphenols, flavonoids levels and antioxidant activity were higher in camu camu (p &lt; 0.001) than in black maca. In conclusion, M. dubia (camu camu) has potential effects improving spermatogenesis and co-administered with maca increase stages of mitosis, meiosis and spermiation of the spermatogenic cycle as assessed by the transillumination technique. This technique is becoming increasingly a useful tool for assessment spermatogenesis.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.934021
The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats
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