Pvmsp8 as a novel plasmodium vivax malaria sero-marker for the peruvian amazon

• Autores: Villasis E., Garro K., Rosas-Aguirre A., Rodriguez P., Rosado J., Gave A., Guzman-Guzman M., Manrique P., White M., Speybroeck N., Vinetz J.M., Torres K., Gamboa D.
• Formato: artículo
• Fecha de Publicación: 2021
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/2375
• Enlace del recurso: https://hdl.handle.net/20.500.12390/2375; https://doi.org/10.3390/pathogens10030282
• Nivel de acceso: acceso abierto
• Materia: PvMSP8; Antibodies; ELISA; Luminex; Malaria; P. vivax; http://purl.org/pe-repo/ocde/ford#3.03.09

The measurement of recent malaria exposure can support malaria control efforts. This study evaluated serological responses to an in-house Plasmodium vivax Merozoite Surface Protein 8 (PvMSP8) expressed in a Baculovirus system as sero-marker of recent exposure to P. vivax (Pv) in the Peruvian Amazon. In a first evaluation, IgGs against PvMSP8 and PvMSP10 proteins were measured by Luminex in a cohort of 422 Amazonian individuals with known history of Pv exposure (monthly data of infection status by qPCR and/or microscopy over five months). Both serological responses were able to discriminate between exposed and non-exposed individuals in a good manner, with slightly higher performance of anti-PvMSP10 IgGs (area under the curve AUC = 0.78 [95% CI = 0.72–0.83]) than anti-PvMSP8 IgGs (AUC = 0.72 [95% CI = 0.67–0.78]) (p = 0.01). In a second evaluation, the analysis by ELISA of 1251 plasma samples, collected during a population-based cross-sectional survey, confirmed the good performance of anti-PvMSP8 IgGs for discriminating between individuals with Pv infection at the time of survey and/or with antecedent of Pv in the past month (AUC = 0.79 [95% CI = 0.74–0.83]). Anti-PvMSP8 IgG antibodies can be considered as a good biomarker of recent Pv exposure in low-moderate transmission settings of the Peruvian Amazon. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.