A novel inexpensive electrochemical sensor for pyrazinoic acid as a potential tool for the identification of pyrazinamide-resistant Mycobacterium tuberculosis

• Autores: Rueda D., Furukawa R., Fuentes P., Comina G., Rey De Castro N.G., Requena D., Gilman R.H., Sheen P., Zimic M.
• Formato: artículo
• Fecha de Publicación: 2018
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/495
• Enlace del recurso: https://hdl.handle.net/20.500.12390/495; https://doi.org/10.4103/ijmy.ijmy_63_18
• Nivel de acceso: acceso abierto
• Materia: water; gold; platinum; pyrazinamide; pyrazinoic acid; tuberculostatic agent; antibiotic resistance; Article; bacterium identification; concentration (parameters); controlled study; cyclic potentiometry; electric current; Mycobacterium tuberculosis; nonhuman; priority journal; supernatant; analogs and derivatives; culture medium; drug effect; electrochemical analysis; https://purl.org/pe-repo/ocde/ford#3.03.00

Tuberculosis (TB) is a significant cause of morbidity and mortality worldwide. The patient compliance with the long treatment regimens is essential for successful eradication. Pyrazinamide (PZA) shortens these regimens from 9 to 6 months, and therefore, improves treatment completion rates. Although PZA is a first-line medication for the treatment of TB, no simple or reliable assay to determine PZA resistance is yet available. In the presence of PZA, only susceptible Mycobacterium tuberculosis strains release pyrazinoic acid (POA). Therefore, the measurement and quantification of released POA is an indicator of PZA resistance.