Characterization of the gene sequence of the peptide transport protein (PepT1) in paiche Arapaima gigas fingerlings [Caracterización de la secuencia del gen de la proteín transportadora de péptidos (PepT1) en alevines de paiche Arapaima gigas]

• Autores: Toledo O.E., Castañeda A.E., Feria M.A., Masías P., Cueva M., Motte E.
• Formato: artículo
• Fecha de Publicación: 2019
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/2739
• Enlace del recurso: https://hdl.handle.net/20.500.12390/2739; https://doi.org/10.15381/rivep.v30i3.15400
• Nivel de acceso: acceso abierto
• Materia: Proteomic; Arapaima gigas; MALDI TOF/TOF; Mass spectrometry; PepT1; Peptide transporter; http://purl.org/pe-repo/ocde/ford#3.02.27

The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression. © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved.