Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin

Descripción del Articulo

The authors acknowledge the CONCYTEC from Peru for the attribution of a master grant to one of us to realize part of the studies.
Detalles Bibliográficos
Autores: Guzman, J, Tene, N, Touchard, A, Castillo, D, Belkhelfa, H, Haddioui-Hbabi, L, Treilhou, M, Sauvain, M
Formato: artículo
Fecha de Publicación:2018
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/1195
Enlace del recurso:https://hdl.handle.net/20.500.12390/1195
https://doi.org/10.3390/toxins10010021
Nivel de acceso:acceso abierto
Materia:SEM
Helicobacter pylori
antimicrobial peptide
bacterial adhesion
bicarinalin
gastric cells
https://purl.org/pe-repo/ocde/ford#1.04.00
id CONC_3fed03bbb8a8d4f004e02b185c0353bf
oai_identifier_str oai:repositorio.concytec.gob.pe:20.500.12390/1195
network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
spellingShingle Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
Guzman, J
SEM
Helicobacter pylori
antimicrobial peptide
bacterial adhesion
bicarinalin
gastric cells
https://purl.org/pe-repo/ocde/ford#1.04.00
title_short Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_full Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_fullStr Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_full_unstemmed Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
title_sort Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
author Guzman, J
author_facet Guzman, J
Tene, N
Touchard, A
Castillo, D
Belkhelfa, H
Haddioui-Hbabi, L
Treilhou, M
Sauvain, M
author_role author
author2 Tene, N
Touchard, A
Castillo, D
Belkhelfa, H
Haddioui-Hbabi, L
Treilhou, M
Sauvain, M
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guzman, J
Tene, N
Touchard, A
Castillo, D
Belkhelfa, H
Haddioui-Hbabi, L
Treilhou, M
Sauvain, M
dc.subject.none.fl_str_mv SEM
topic SEM
Helicobacter pylori
antimicrobial peptide
bacterial adhesion
bicarinalin
gastric cells
https://purl.org/pe-repo/ocde/ford#1.04.00
dc.subject.es_PE.fl_str_mv Helicobacter pylori
antimicrobial peptide
bacterial adhesion
bicarinalin
gastric cells
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#1.04.00
description The authors acknowledge the CONCYTEC from Peru for the attribution of a master grant to one of us to realize part of the studies.
publishDate 2018
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/1195
dc.identifier.doi.none.fl_str_mv https://doi.org/10.3390/toxins10010021
dc.identifier.isi.none.fl_str_mv 424096500021
url https://hdl.handle.net/20.500.12390/1195
https://doi.org/10.3390/toxins10010021
identifier_str_mv 424096500021
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv TOXINS
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.none.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1839175749303533568
spelling Publicationrp03412600rp03411600rp03408600rp01884500rp03413600rp03409600rp03410600rp01882500Guzman, JTene, NTouchard, ACastillo, DBelkhelfa, HHaddioui-Hbabi, LTreilhou, MSauvain, M2024-05-30T23:13:38Z2024-05-30T23:13:38Z2018https://hdl.handle.net/20.500.12390/1195https://doi.org/10.3390/toxins10010021424096500021The authors acknowledge the CONCYTEC from Peru for the attribution of a master grant to one of us to realize part of the studies.The venom peptide bicarinalin, previously isolated from the ant Tetramorium bicarinatum, is an antimicrobial agent with a broad spectrum of activity. In this study, we investigate the potential of bicarinalin as a novel agent against Helicobacter pylori, which causes several gastric diseases. First, the effects of synthetic bicarinalin have been tested against Helicobacter pylori: one ATCC strain, and forty-four isolated from stomach ulcer biopsies of Peruvian patients. Then the cytoxicity of bicarinalin on human gastric cells and murine peritoneal macrophages was measured using XTT and MTT assays, respectively. Finally, the preventive effect of bicarinalin was evaluated by scanning electron microscopy using an adherence assay of H. pylori on human gastric cells treated with bicarinalin. This peptide has a potent antibacterial activity at the same magnitude as four antibiotics currently used in therapies against H. pylori. Bicarinalin also inhibited adherence of H. pylori to gastric cells with an IC50 of 0.12 μg·mL−1 and had low toxicity for human cells. Scanning electron microscopy confirmed that bicarinalin can significantly decrease the density of H. pylori on gastric cells. We conclude that Bicarinalin is a promising compound for the development of a novel and effective anti-H. pylori agent for both curative and preventive use.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengMDPITOXINSinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/SEMHelicobacter pylori-1antimicrobial peptide-1bacterial adhesion-1bicarinalin-1gastric cells-1https://purl.org/pe-repo/ocde/ford#1.04.00-1Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalininfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#20.500.12390/1195oai:repositorio.concytec.gob.pe:20.500.12390/11952024-05-30 15:36:36.033http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="ca937468-a72b-4e4b-bc04-5bc6b2b78669"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin</Title> <PublishedIn> <Publication> <Title>TOXINS</Title> </Publication> </PublishedIn> <PublicationDate>2018</PublicationDate> <DOI>https://doi.org/10.3390/toxins10010021</DOI> <ISI-Number>424096500021</ISI-Number> <Authors> <Author> <DisplayName>Guzman, J</DisplayName> <Person id="rp03412" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Tene, N</DisplayName> <Person id="rp03411" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Touchard, A</DisplayName> <Person id="rp03408" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Castillo, D</DisplayName> <Person id="rp01884" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Belkhelfa, H</DisplayName> <Person id="rp03413" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Haddioui-Hbabi, L</DisplayName> <Person id="rp03409" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Treilhou, M</DisplayName> <Person id="rp03410" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Sauvain, M</DisplayName> <Person id="rp01882" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>MDPI</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>http://creativecommons.org/licenses/by-nc/4.0/</License> <Keyword>SEM</Keyword> <Keyword>Helicobacter pylori</Keyword> <Keyword>antimicrobial peptide</Keyword> <Keyword>bacterial adhesion</Keyword> <Keyword>bicarinalin</Keyword> <Keyword>gastric cells</Keyword> <Abstract>The venom peptide bicarinalin, previously isolated from the ant Tetramorium bicarinatum, is an antimicrobial agent with a broad spectrum of activity. In this study, we investigate the potential of bicarinalin as a novel agent against Helicobacter pylori, which causes several gastric diseases. First, the effects of synthetic bicarinalin have been tested against Helicobacter pylori: one ATCC strain, and forty-four isolated from stomach ulcer biopsies of Peruvian patients. Then the cytoxicity of bicarinalin on human gastric cells and murine peritoneal macrophages was measured using XTT and MTT assays, respectively. Finally, the preventive effect of bicarinalin was evaluated by scanning electron microscopy using an adherence assay of H. pylori on human gastric cells treated with bicarinalin. This peptide has a potent antibacterial activity at the same magnitude as four antibiotics currently used in therapies against H. pylori. Bicarinalin also inhibited adherence of H. pylori to gastric cells with an IC50 of 0.12 μg·mL−1 and had low toxicity for human cells. Scanning electron microscopy confirmed that bicarinalin can significantly decrease the density of H. pylori on gastric cells. We conclude that Bicarinalin is a promising compound for the development of a novel and effective anti-H. pylori agent for both curative and preventive use.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.24362
Anti-Helicobacter pylori Properties of the Ant-Venom Peptide Bicarinalin
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

Ejemplares Similares