A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure
Descripción del Articulo
Especial thanks to Dr. James Higgins for statistical consultation and to Mrs. Jacque Staat and Mrs. Megan Haugh for the manuscript review. This work was supported by the International Foundation of Science IFS Stockholm Sweden, Grant B:2083-2 and the Consejo Nacional de Ciencia y Tecnologia CONCYTEC...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2007 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/731 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/731 https://doi.org/10.1016/j.smallrumres.2006.04.017 |
| Nivel de acceso: | acceso abierto |
| Materia: | Lama pacos Animalia Bacteria (microorganisms) Corynebacterium pseudotuberculosis https://purl.org/pe-repo/ocde/ford#4.03.00 |
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| dc.title.none.fl_str_mv |
A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure |
| title |
A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure |
| spellingShingle |
A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure Braga W. Lama pacos Animalia Bacteria (microorganisms) Corynebacterium pseudotuberculosis https://purl.org/pe-repo/ocde/ford#4.03.00 |
| title_short |
A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure |
| title_full |
A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure |
| title_fullStr |
A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure |
| title_full_unstemmed |
A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure |
| title_sort |
A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure |
| author |
Braga W. |
| author_facet |
Braga W. Schul S. Nuñez A. Pezo D. Franco E. |
| author_role |
author |
| author2 |
Schul S. Nuñez A. Pezo D. Franco E. |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Braga W. Schul S. Nuñez A. Pezo D. Franco E. |
| dc.subject.none.fl_str_mv |
Lama pacos |
| topic |
Lama pacos Animalia Bacteria (microorganisms) Corynebacterium pseudotuberculosis https://purl.org/pe-repo/ocde/ford#4.03.00 |
| dc.subject.es_PE.fl_str_mv |
Animalia Bacteria (microorganisms) Corynebacterium pseudotuberculosis |
| dc.subject.ocde.none.fl_str_mv |
https://purl.org/pe-repo/ocde/ford#4.03.00 |
| description |
Especial thanks to Dr. James Higgins for statistical consultation and to Mrs. Jacque Staat and Mrs. Megan Haugh for the manuscript review. This work was supported by the International Foundation of Science IFS Stockholm Sweden, Grant B:2083-2 and the Consejo Nacional de Ciencia y Tecnologia CONCYTEC, Lima, Peru. |
| publishDate |
2007 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2007 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/731 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.smallrumres.2006.04.017 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-34547764934 |
| url |
https://hdl.handle.net/20.500.12390/731 https://doi.org/10.1016/j.smallrumres.2006.04.017 |
| identifier_str_mv |
2-s2.0-34547764934 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Small Ruminant Research |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
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Consejo Nacional de Ciencia Tecnología e Innovación |
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CONCYTEC |
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CONCYTEC |
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CONCYTEC-Institucional |
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CONCYTEC-Institucional |
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Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1844883090921488384 |
| spelling |
Publicationrp01852600rp01850600rp01848600rp01851600rp01849600Braga W.Schul S.Nuñez A.Pezo D.Franco E.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2007https://hdl.handle.net/20.500.12390/731https://doi.org/10.1016/j.smallrumres.2006.04.0172-s2.0-34547764934Especial thanks to Dr. James Higgins for statistical consultation and to Mrs. Jacque Staat and Mrs. Megan Haugh for the manuscript review. This work was supported by the International Foundation of Science IFS Stockholm Sweden, Grant B:2083-2 and the Consejo Nacional de Ciencia y Tecnologia CONCYTEC, Lima, Peru.Corynebacterium pseudotuberculosis is the agent of alpaca's lymphadenitis. The present study was to demonstrate the effect of a primary infection with low (1.1 × 103), moderate (1 × 104), and high (1.2 × 105) doses of C. pseudotuberculosis against a significant higher challenge dose of 9 × 108 CFU of C. pseudotuberculosis. Three groups of 4 healthy male alpacas were inoculated subcutaneously (SC) in the left flank behind the costal arch with the above doses of bacteria. A fourth group of 4 alpacas was sham inoculated with phosphate buffered saline as control. After 5 weeks all animals were challenged with a dose of 9 × 108 CFU of C. pseudotuberculosis inoculated SC in the right flank. The alpacas were clinically inspected for local and regional abscesses, body temperature and behavior changes. The primary infected alpacas had a febrile response, and abscesses at the inoculation point and regional lymph nodes. However, after challenge, the primary infected animals showed no superficial lesions or febrile response. In contrast, the immune naïve alpacas from group D developed a severe disease characterized by fever, abscesses in regional lymphnodes, and in one alpaca a subcutaneous edema and sudden death 2 weeks after exposure. In addition, primary infected alpacas had a robust antibody response against C. pseudotuberculosis cell wall antigen with significant differences with respect the naïve challenged alpacas. At necropsy, the primary infected alpacas had abscesses only in the regional or internal renal-lymph nodes from the left or primary inoculation side of the body, with no lesions in the right challenged side. In contrast, the primary sham inoculated alpacas had abscesses in the regional and internal lymph nodes from the right challenged side. This work showed that a primary infection with at least 1.1 × 103 viable C. pseudotuberculosis induces protection against a second high dose exposure to this bacterium. These results will be useful for further study of prevention methods to control lymphadenitis in alpacas.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengElsevierSmall Ruminant Researchinfo:eu-repo/semantics/openAccessLama pacosAnimalia-1Bacteria (microorganisms)-1Corynebacterium pseudotuberculosis-1https://purl.org/pe-repo/ocde/ford#4.03.00-1A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposureinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/731oai:repositorio.concytec.gob.pe:20.500.12390/7312024-05-30 15:58:49.46http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="4a27d9ed-c164-419b-a075-ef79d5471039"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>A primary Corynebacterium pseudotuberculosis low dose infection in alpacas (Lama pacos) protects against a lethal challenge exposure</Title> <PublishedIn> <Publication> <Title>Small Ruminant Research</Title> </Publication> </PublishedIn> <PublicationDate>2007</PublicationDate> <DOI>https://doi.org/10.1016/j.smallrumres.2006.04.017</DOI> <SCP-Number>2-s2.0-34547764934</SCP-Number> <Authors> <Author> <DisplayName>Braga W.</DisplayName> <Person id="rp01852" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Schul S.</DisplayName> <Person id="rp01850" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Nuñez A.</DisplayName> <Person id="rp01848" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Pezo D.</DisplayName> <Person id="rp01851" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Franco E.</DisplayName> <Person id="rp01849" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Elsevier</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Lama pacos</Keyword> <Keyword>Animalia</Keyword> <Keyword>Bacteria (microorganisms)</Keyword> <Keyword>Corynebacterium pseudotuberculosis</Keyword> <Abstract>Corynebacterium pseudotuberculosis is the agent of alpaca's lymphadenitis. The present study was to demonstrate the effect of a primary infection with low (1.1 × 103), moderate (1 × 104), and high (1.2 × 105) doses of C. pseudotuberculosis against a significant higher challenge dose of 9 × 108 CFU of C. pseudotuberculosis. Three groups of 4 healthy male alpacas were inoculated subcutaneously (SC) in the left flank behind the costal arch with the above doses of bacteria. A fourth group of 4 alpacas was sham inoculated with phosphate buffered saline as control. After 5 weeks all animals were challenged with a dose of 9 × 108 CFU of C. pseudotuberculosis inoculated SC in the right flank. The alpacas were clinically inspected for local and regional abscesses, body temperature and behavior changes. The primary infected alpacas had a febrile response, and abscesses at the inoculation point and regional lymph nodes. However, after challenge, the primary infected animals showed no superficial lesions or febrile response. In contrast, the immune naïve alpacas from group D developed a severe disease characterized by fever, abscesses in regional lymphnodes, and in one alpaca a subcutaneous edema and sudden death 2 weeks after exposure. In addition, primary infected alpacas had a robust antibody response against C. pseudotuberculosis cell wall antigen with significant differences with respect the naïve challenged alpacas. At necropsy, the primary infected alpacas had abscesses only in the regional or internal renal-lymph nodes from the left or primary inoculation side of the body, with no lesions in the right challenged side. In contrast, the primary sham inoculated alpacas had abscesses in the regional and internal lymph nodes from the right challenged side. This work showed that a primary infection with at least 1.1 × 103 viable C. pseudotuberculosis induces protection against a second high dose exposure to this bacterium. These results will be useful for further study of prevention methods to control lymphadenitis in alpacas.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
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13.413335 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
