Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease
Descripción del Articulo
The study was supported by the Programa Nacional de Innovaci?n para la Competitividad y Productividad (Inn?vate Per?), under the contract 117-PNICP-PIAP-2015. J.R. had a fellowship from the program I3, of the ISCIII [grant number: CES11/012]. C.G. had a PhD fellowship of the ISCIII [FI12/00561] and...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2021 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/3012 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/3012 https://doi.org/10.3390/pathogens10080917 |
| Nivel de acceso: | acceso abierto |
| Materia: | Peptides Bartonella bacilliformis Carrion’s disease Carrion’s disease Diagnostic ELISA Immunogenic tools https://purl.org/pe-repo/ocde/ford#3.03.08 |
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CONC |
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CONCYTEC-Institucional |
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4689 |
| dc.title.none.fl_str_mv |
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease |
| title |
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease |
| spellingShingle |
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease Gomes C. Peptides Bartonella bacilliformis Bartonella bacilliformis Carrion’s disease Carrion’s disease Diagnostic Diagnostic ELISA Immunogenic tools Immunogenic tools https://purl.org/pe-repo/ocde/ford#3.03.08 |
| title_short |
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease |
| title_full |
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease |
| title_fullStr |
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease |
| title_full_unstemmed |
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease |
| title_sort |
Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease |
| author |
Gomes C. |
| author_facet |
Gomes C. Pons M.J. Del Valle-Mendoza J. Matsuoka M. Ruiz J. |
| author_role |
author |
| author2 |
Pons M.J. Del Valle-Mendoza J. Matsuoka M. Ruiz J. |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Gomes C. Pons M.J. Del Valle-Mendoza J. Matsuoka M. Ruiz J. |
| dc.subject.none.fl_str_mv |
Peptides |
| topic |
Peptides Bartonella bacilliformis Bartonella bacilliformis Carrion’s disease Carrion’s disease Diagnostic Diagnostic ELISA Immunogenic tools Immunogenic tools https://purl.org/pe-repo/ocde/ford#3.03.08 |
| dc.subject.es_PE.fl_str_mv |
Bartonella bacilliformis Bartonella bacilliformis Carrion’s disease Carrion’s disease Diagnostic Diagnostic ELISA Immunogenic tools Immunogenic tools |
| dc.subject.ocde.none.fl_str_mv |
https://purl.org/pe-repo/ocde/ford#3.03.08 |
| description |
The study was supported by the Programa Nacional de Innovaci?n para la Competitividad y Productividad (Inn?vate Per?), under the contract 117-PNICP-PIAP-2015. J.R. had a fellowship from the program I3, of the ISCIII [grant number: CES11/012]. C.G. had a PhD fellowship of the ISCIII [FI12/00561] and was recipient of a Canon Foundation Fellowship and a Small Grant from the Royal Society for Tropical Medicine and Hygiene [grant reference 000584]. MJP had a postdoctoral fellowship from CONCYTEC/FONDECYT [grant number: CG05-2013-FONDECYT]. |
| publishDate |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/3012 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.3390/pathogens10080917 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-85111660194 |
| url |
https://hdl.handle.net/20.500.12390/3012 https://doi.org/10.3390/pathogens10080917 |
| identifier_str_mv |
2-s2.0-85111660194 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Pathogens |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.uri.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
| instname_str |
Consejo Nacional de Ciencia Tecnología e Innovación |
| instacron_str |
CONCYTEC |
| institution |
CONCYTEC |
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CONCYTEC-Institucional |
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CONCYTEC-Institucional |
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Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1839175424009043968 |
| spelling |
Publicationrp01359600rp01161600rp06631600rp06551600rp01358600Gomes C.Pons M.J.Del Valle-Mendoza J.Matsuoka M.Ruiz J.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2021https://hdl.handle.net/20.500.12390/3012https://doi.org/10.3390/pathogens100809172-s2.0-85111660194The study was supported by the Programa Nacional de Innovaci?n para la Competitividad y Productividad (Inn?vate Per?), under the contract 117-PNICP-PIAP-2015. J.R. had a fellowship from the program I3, of the ISCIII [grant number: CES11/012]. C.G. had a PhD fellowship of the ISCIII [FI12/00561] and was recipient of a Canon Foundation Fellowship and a Small Grant from the Royal Society for Tropical Medicine and Hygiene [grant reference 000584]. MJP had a postdoctoral fellowship from CONCYTEC/FONDECYT [grant number: CG05-2013-FONDECYT].Bartonella bacilliformis is the causal agent of Carrion’s disease, an overlooked illness endemic in the Andean Mountains with Peru being the most affected country. The diagnostic of this illness is a challenge due to the limited resources and the common symptomatology with other infectious diseases. The goal of this study was to identify immunogenic peptides from Pap31 and succinyl-CoA synthetase ? (SCS-?) of B. bacilliformis that might be suitable for developing a serologic tool. The immunodominant character of Pap31 and SCS-? was determined byWestern blotting and in-silico analysis. Subsequently, 35 peptides were selected for epitope mapping and their immunoreactivity was tested by enzyme-linked immunosorbent assay (ELISA). A total of 30 sera were tested including pre-exposed people with high IgM levels for Pap31/SCS-? (23 sera), patients (2 sera) as well as 5 sera with no reactivity to Pap31/SCS-?. The results indicate that Pap31-8 (187QAIGSAILKGTKDTGT202) and SCS-?-12 (59IFASVAEGKEKTGANA74) are the most immunogenic peptides, with Pap31-8 showing potential to discriminate between B. bacilliformis and the remaining Bartonella spp., and SCS-?-12 differentiating Bartonella spp. from other microorganisms. © 2021 by the authors.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengMDPIPathogensinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/PeptidesBartonella bacilliformis-1Bartonella bacilliformis-1Carrion’s disease-1Carrion’s disease-1Diagnostic-1Diagnostic-1ELISA-1Immunogenic tools-1Immunogenic tools-1https://purl.org/pe-repo/ocde/ford#3.03.08-1Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s diseaseinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/3012oai:repositorio.concytec.gob.pe:20.500.12390/30122024-05-30 16:13:07.991https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="134909a2-a5cb-4434-8286-933bdfbd078b"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Immunogenic peptides from Pap31 and SCS-? of Bartonella bacilliformis: One step closer to a rapid diagnostic tool for Carrion’s disease</Title> <PublishedIn> <Publication> <Title>Pathogens</Title> </Publication> </PublishedIn> <PublicationDate>2021</PublicationDate> <DOI>https://doi.org/10.3390/pathogens10080917</DOI> <SCP-Number>2-s2.0-85111660194</SCP-Number> <Authors> <Author> <DisplayName>Gomes C.</DisplayName> <Person id="rp01359" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Pons M.J.</DisplayName> <Person id="rp01161" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Del Valle-Mendoza J.</DisplayName> <Person id="rp06631" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Matsuoka M.</DisplayName> <Person id="rp06551" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Ruiz J.</DisplayName> <Person id="rp01358" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>MDPI</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by-nc-nd/4.0/</License> <Keyword>Peptides</Keyword> <Keyword>Bartonella bacilliformis</Keyword> <Keyword>Bartonella bacilliformis</Keyword> <Keyword>Carrion’s disease</Keyword> <Keyword>Carrion’s disease</Keyword> <Keyword>Diagnostic</Keyword> <Keyword>Diagnostic</Keyword> <Keyword>ELISA</Keyword> <Keyword>Immunogenic tools</Keyword> <Keyword>Immunogenic tools</Keyword> <Abstract>Bartonella bacilliformis is the causal agent of Carrion’s disease, an overlooked illness endemic in the Andean Mountains with Peru being the most affected country. The diagnostic of this illness is a challenge due to the limited resources and the common symptomatology with other infectious diseases. The goal of this study was to identify immunogenic peptides from Pap31 and succinyl-CoA synthetase ? (SCS-?) of B. bacilliformis that might be suitable for developing a serologic tool. The immunodominant character of Pap31 and SCS-? was determined byWestern blotting and in-silico analysis. Subsequently, 35 peptides were selected for epitope mapping and their immunoreactivity was tested by enzyme-linked immunosorbent assay (ELISA). A total of 30 sera were tested including pre-exposed people with high IgM levels for Pap31/SCS-? (23 sera), patients (2 sera) as well as 5 sera with no reactivity to Pap31/SCS-?. The results indicate that Pap31-8 (187QAIGSAILKGTKDTGT202) and SCS-?-12 (59IFASVAEGKEKTGANA74) are the most immunogenic peptides, with Pap31-8 showing potential to discriminate between B. bacilliformis and the remaining Bartonella spp., and SCS-?-12 differentiating Bartonella spp. from other microorganisms. © 2021 by the authors.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
| score |
13.430107 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
