Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines

Descripción del Articulo

This work was supported by Proyecto CONCYTEC–Banco Mundial “Mejoramiento y Ampliación de los Servicios del Sistema Nacional de Ciencia Tecnología e Innovación Tecnológica” 8682-PE ( 104-2018-FONDECYT-BM-IADT-AV ).
Detalles Bibliográficos
Autores: Enciso-Benavides J., Alfaro L., Castañeda-Altamirano C., Rojas, N., González-Cabeza J., Enciso N., Riesco F., Castillo M., Enciso J.
Formato: artículo
Fecha de Publicación:2021
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/2977
Enlace del recurso:https://hdl.handle.net/20.500.12390/2977
https://doi.org/10.1016/j.heliyon.2021.e07273
Nivel de acceso:acceso abierto
Materia:Triple-negative breast tumors
Cancer stem cells
CD44high/CD24low phenotype cells
Magnetic immunoselection
https://purl.org/pe-repo/ocde/ford#3.04.02
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network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
title Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
spellingShingle Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
Enciso-Benavides J.
Triple-negative breast tumors
Cancer stem cells
Cancer stem cells
CD44high/CD24low phenotype cells
CD44high/CD24low phenotype cells
Magnetic immunoselection
Magnetic immunoselection
https://purl.org/pe-repo/ocde/ford#3.04.02
title_short Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
title_full Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
title_fullStr Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
title_full_unstemmed Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
title_sort Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
author Enciso-Benavides J.
author_facet Enciso-Benavides J.
Alfaro L.
Castañeda-Altamirano C.
Rojas, N.
González-Cabeza J.
Enciso N.
Riesco F.
Castillo M.
Enciso J.
author_role author
author2 Alfaro L.
Castañeda-Altamirano C.
Rojas, N.
González-Cabeza J.
Enciso N.
Riesco F.
Castillo M.
Enciso J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Enciso-Benavides J.
Alfaro L.
Castañeda-Altamirano C.
Rojas, N.
González-Cabeza J.
Enciso N.
Riesco F.
Castillo M.
Enciso J.
dc.subject.none.fl_str_mv Triple-negative breast tumors
topic Triple-negative breast tumors
Cancer stem cells
Cancer stem cells
CD44high/CD24low phenotype cells
CD44high/CD24low phenotype cells
Magnetic immunoselection
Magnetic immunoselection
https://purl.org/pe-repo/ocde/ford#3.04.02
dc.subject.es_PE.fl_str_mv Cancer stem cells
Cancer stem cells
CD44high/CD24low phenotype cells
CD44high/CD24low phenotype cells
Magnetic immunoselection
Magnetic immunoselection
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#3.04.02
description This work was supported by Proyecto CONCYTEC–Banco Mundial “Mejoramiento y Ampliación de los Servicios del Sistema Nacional de Ciencia Tecnología e Innovación Tecnológica” 8682-PE ( 104-2018-FONDECYT-BM-IADT-AV ).
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/2977
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.heliyon.2021.e07273
dc.identifier.scopus.none.fl_str_mv 2-s2.0-85109559504
url https://hdl.handle.net/20.500.12390/2977
https://doi.org/10.1016/j.heliyon.2021.e07273
identifier_str_mv 2-s2.0-85109559504
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Heliyon
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.none.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
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spelling Publicationrp02493600rp08434600rp08437600rp06429600rp08439600rp08435600rp08436600rp06447600rp08438600Enciso-Benavides J.Alfaro L.Castañeda-Altamirano C.Rojas, N.González-Cabeza J.Enciso N.Riesco F.Castillo M.Enciso J.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2021https://hdl.handle.net/20.500.12390/2977https://doi.org/10.1016/j.heliyon.2021.e072732-s2.0-85109559504This work was supported by Proyecto CONCYTEC–Banco Mundial “Mejoramiento y Ampliación de los Servicios del Sistema Nacional de Ciencia Tecnología e Innovación Tecnológica” 8682-PE ( 104-2018-FONDECYT-BM-IADT-AV ).Triple-negative breast tumours (TNBTs) make up 15–20% of all breast tumours. There is no treatment for them, and the role that cancer stem cells (CSCs) have in carcinogenesis is still unclear, so finding markers and therapeutic targets in CSC exosomes requires these cells to exist as a homogeneous cell population. The objective of this work was to determine differences in ultrastructural morphology, proliferative capacity, and mouse-xenotransplantation characteristics of the MDA-MB-231 and MDA-MB-436 TNBT cell lines with the CD44high/CD24low phenotype in order to study their exosomes. The results show that the CD44high/CD24low MBA-MB-231 cells had a population doubling time of 41.56 h, compared to 44.79 h in the MDA-MB-436 cell line. After magnetic immunoseparation, 18.75% and 14.56% of the stem cell population of the MDA-MB-231 and MDA-MB-436 cell lines, respectively, were of the CD44high/CD24low phenotype, which were expanded to reach purities of 80.4% and 87.6%. The same expanded lineage in both cell lines was shown to possess the pluripotency markers Nanog and Oct4. Under a scanning electron microscope, the CD44high/CD24low lineage of the MBA-MD-231 cell line formed groups of more interconnected cells than this lineage of the MBA-MD-436 line. A total of 16% of the mice inoculated with the CD44high/CD24low lineage of either cell line presented tumours of the breast, lung, and submandibular ganglia, in whose tissues variable numbers of inoculated cells were found 30 days post-inoculation. By magnetic immunoselection, it was possible to isolate in similar quantities and characterize, expand, and xenotransplant the CD44high/CD24low lineage of the MDA-MB-231 and MDA-MB-436 cell lines. The former cell line has greater proliferative capacity, the two lines differ under scanning electron microscopy in how they intercommunicate, and both cell lines induce new tumours in mice and persist at least 30 days post-inoculation in the transplanted animal so their exosomes would also be different. © 2021 The Author(s)Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengElsevier LtdHeliyoninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/Triple-negative breast tumorsCancer stem cells-1Cancer stem cells-1CD44high/CD24low phenotype cells-1CD44high/CD24low phenotype cells-1Magnetic immunoselection-1Magnetic immunoselection-1https://purl.org/pe-repo/ocde/ford#3.04.02-1Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell linesinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/2977oai:repositorio.concytec.gob.pe:20.500.12390/29772024-05-30 16:12:44.327https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="59514839-d68b-4a7e-9bed-471878e2360e"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines</Title> <PublishedIn> <Publication> <Title>Heliyon</Title> </Publication> </PublishedIn> <PublicationDate>2021</PublicationDate> <DOI>https://doi.org/10.1016/j.heliyon.2021.e07273</DOI> <SCP-Number>2-s2.0-85109559504</SCP-Number> <Authors> <Author> <DisplayName>Enciso-Benavides J.</DisplayName> <Person id="rp02493" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Alfaro L.</DisplayName> <Person id="rp08434" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Castañeda-Altamirano C.</DisplayName> <Person id="rp08437" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Rojas, N.</DisplayName> <Person id="rp06429" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>González-Cabeza J.</DisplayName> <Person id="rp08439" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Enciso N.</DisplayName> <Person id="rp08435" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Riesco F.</DisplayName> <Person id="rp08436" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Castillo M.</DisplayName> <Person id="rp06447" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Enciso J.</DisplayName> <Person id="rp08438" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Elsevier Ltd</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by-nc-nd/4.0/</License> <Keyword>Triple-negative breast tumors</Keyword> <Keyword>Cancer stem cells</Keyword> <Keyword>Cancer stem cells</Keyword> <Keyword>CD44high/CD24low phenotype cells</Keyword> <Keyword>CD44high/CD24low phenotype cells</Keyword> <Keyword>Magnetic immunoselection</Keyword> <Keyword>Magnetic immunoselection</Keyword> <Abstract>Triple-negative breast tumours (TNBTs) make up 15–20% of all breast tumours. There is no treatment for them, and the role that cancer stem cells (CSCs) have in carcinogenesis is still unclear, so finding markers and therapeutic targets in CSC exosomes requires these cells to exist as a homogeneous cell population. The objective of this work was to determine differences in ultrastructural morphology, proliferative capacity, and mouse-xenotransplantation characteristics of the MDA-MB-231 and MDA-MB-436 TNBT cell lines with the CD44high/CD24low phenotype in order to study their exosomes. The results show that the CD44high/CD24low MBA-MB-231 cells had a population doubling time of 41.56 h, compared to 44.79 h in the MDA-MB-436 cell line. After magnetic immunoseparation, 18.75% and 14.56% of the stem cell population of the MDA-MB-231 and MDA-MB-436 cell lines, respectively, were of the CD44high/CD24low phenotype, which were expanded to reach purities of 80.4% and 87.6%. The same expanded lineage in both cell lines was shown to possess the pluripotency markers Nanog and Oct4. Under a scanning electron microscope, the CD44high/CD24low lineage of the MBA-MD-231 cell line formed groups of more interconnected cells than this lineage of the MBA-MD-436 line. A total of 16% of the mice inoculated with the CD44high/CD24low lineage of either cell line presented tumours of the breast, lung, and submandibular ganglia, in whose tissues variable numbers of inoculated cells were found 30 days post-inoculation. By magnetic immunoselection, it was possible to isolate in similar quantities and characterize, expand, and xenotransplant the CD44high/CD24low lineage of the MDA-MB-231 and MDA-MB-436 cell lines. The former cell line has greater proliferative capacity, the two lines differ under scanning electron microscopy in how they intercommunicate, and both cell lines induce new tumours in mice and persist at least 30 days post-inoculation in the transplanted animal so their exosomes would also be different. © 2021 The Author(s)</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.413352
Biological characteristics of a sub-population of cancer stem cells from two triple-negative breast tumour cell lines
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