Genotype identification of Staphylococcus with methicillin-resistant phenotype isolated from human, animal and environmental samples

• Autores: Espejo, Luis José, Rodríguez, Karen Lorena, Rodríguez, Martha Fabiola, Gómez Ramírez, Arlen Patricia
• Formato: artículo
• Fecha de Publicación: 2019
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/15947
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/15947
• Nivel de acceso: acceso abierto
• Materia: mecA; drug resistance; Staphylococcus spp; veterinary hospital; mecA; resistencia antimicrobiana; Staphylococcus spp; hospital veterinario

The aim of this study was to identify the mecA-1 gene of Staphylococcus isolates with methicillin-resistant phenotype (SMR) obtained from biological samples and surfaces of a veterinary clinic. Nine isolates with SMR phenotype classified by the automated VITEK system were selected. The genus and the variability were corroborated by amplification and sequencing of the 16S rRNA and tuf genes. The presence of the mecA- 1 gene was also established by PCR. In the nine isolates with SMR phenotype, the 16S rRNA and tuf genes were identified, showing 99% similarity with reference strains; however, only in five isolates was found the mecA-1 gene. The results showed a greater identification of the MR genotype in intra-hospital surfaces, which indicates the need for control and monitoring of these strains in veterinary hospitals due to the implications they have on public health.