The cystic echinococcosis, caused by Echinococcus granulosus, is endemic in southern Brazil, especially in the state of Rio Grande do Sul (RS). Recent advances in imaging technology and in immunological tests provide powerful and non-invasive tools for its diagnosis based on the identification of cysts and detection of antibodies. We analyzed the serological profile of the genus Echinococcus by immunoblot in sera from patients from southern of Brazil who had been referred to the National reference service in hydatid disease between January 2010 and July 2013. Among 160 sera samples, 16 were reactive for at least one of the proteins (10, 18, 28, and 40 kDa), indicating that the pattern of reactivity can vary among the patients.