El estudio tuvo como objetivo identificar y caracterizar molecularmente la fauna submareal de la isla Santa mediante el gen mitocondrial COI, con el propósito de actualizar el conocimiento taxonómico y genético de las especies y contribuir al fortalecimiento del plan de manejo de la Reserva Nacional de Islas, Islotes y Puntas Guaneras. En total se colectaron 163 organismos a los que se les extrajo ADN utilizando el método del fenol cloroformo. La calidad y cantidad del ADN se evaluó mediante electroforesis y espectofotometría, respectivamente, y el gen mitocondrial COI fue amplificado por PCR convencional, enviándose los amplicones a secuenciar a Macrogen – USA. Se obtuvieron 105 secuencias consensos de las cuales 88 presentaron una identidad ≥ 99% en el BoldSystem y NCBI. Los análisis de distancia genética (K2P) y delimitación de especies (PTP, bPTP, GMYC y ABGD) confirmar...

In this work, we establish the thermal preference of Cryphiops caementarius adult males, previously acclimated at different temperatures. Shrimp were collected from the Pativilca River. In the laboratory, shrimp were acclimated for 30 days in nine aquariums, each with 10 circular containers. In each container, there was a shrimp (10 per aquarium). Acclimation temperatures were 19, 24 and 28 °C. The gradient temperature system (12 to 33 °C) consisted for a PVC gutter (3 m long and 10 cm deep) with 18 compartments. Submersible thermoregulators (100W) were placed at one end of the gutter and the other end, frozen hydrogel bags. The thermal preference was determined by the acute method and the tests were performed between 08:00 and 14:00 h. Shrimp acclimated at 24 °C moved to compartments whose temperatures were higher than the acclimation temperature. Shrimp acclimated at 24 °C moved to...

In this work, we establish the thermal preference of Cryphiops caementarius adult males, previously acclimated at different temperatures. Shrimp were collected from the Pativilca River. In the laboratory, shrimp were acclimated for 30 days in nine aquariums, each with 10 circular containers. In each container, there was a shrimp (10 per aquarium). Acclimation temperatures were 19, 24 and 28 °C. The gradient temperature system (12 to 33 °C) consisted for a PVC gutter (3 m long and 10 cm deep) with 18 compartments. Submersible thermoregulators (100W) were placed at one end of the gutter and the other end, frozen hydrogel bags. The thermal preference was determined by the acute method and the tests were performed between 08:00 and 14:00 h. Shrimp acclimated at 24 °C moved to compartments whose temperatures were higher than the acclimation temperature. Shrimp acclimated at 24 °C moved to...