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Evalúa el desempeño de cinco métodos fenotípicos para la detección de metalobetalactamasas (MBL) en bacilos gram negativos. Es un estudio observacional, descriptivo y de corte transversal. Utiliza 100 aislamientos de bacilos gram negativos tipificados genotípicamente en estudios preliminares. Los métodos que se evalúan son: Prueba de discos combinados con inhibidor (EDTA), Prueba de sinergia de doble disco, Test de Hodge Modificado (cepa E. coli ATCC 25922), Test de Hodge Modificado (cepa K. pneumoniae ATCC 700603) y Blue Carba Test. De los 32 aislamientos productores de MBL, 27 son del tipo IMP, 3 del tipo VIM y 2 del tipo NDM. De los 68 aislamientos no productores de MBL, 66 son aislamientos no poseedores de MBL, 1 del tipo CTX + impermeabilidad y 1 aislamiento del tipo. El método de discos combinados con inhibidor y el método de sinergia de doble disco son los más específi...

Objetive: Evaluate an Ozone Generating System (OGS) through of the microorganisms count and identificated in the air of the sanitary environments of the National Institute of Health (INS) from Peru. The study: These were exposed to OGS for 1 min per m2, air samples were collected at volumes of 200L, 500L and 1000L by impaction, pre and post exposure to OGS, cultures were obtained on Plate Count Agar (PCA) and Oxytetracycline Glucose Agar (OGA) grown at 35°C±2°C for 48 hours and 25°C ± 2°C for 5 days. Findings: A decrease in the count of fungi after exposure to OGS, the difference being statistically significant for the volumes of 200L (p=0.047) and 1000 L (p<0.005); Aspergillus spp. species were identified in most cultures, in bacteria no significant difference was observed. Conclusions: The OGS is a method that inhibits the growth of most fungi in sanitary environments.