Five strains of Kluyveromyces marxianus a one of Gandida pseudotropicalis were cultured in medium M· 1 for productión of β-galactosidase (E.C: 3:2:1:23). The strain Kluyveromyces marxianus NRRL- Y - 1109 was selecteed for the best production (11.6 U/ml). The maximum yield of enzyme (1330 U ONPG/g of biomass) was obtained on 5% of lactose supplemented with 0.5% yeast extract , 0.75% (NH4)2SO4 y 0.45% K2HPO4 (W/V). The best extraction of the enzyme from viable cells was performed by toluene 2% (v/v), at pH 7.0 ± 0.1, 30ºC during 15 hours of treatment, in 0.1 M potassium phosphate buffer, supplemented with 1 mM magnesium and 0.1 mM manganese sulfate. The enzyme was partially purified with acetone. lt obtained a specific activity of de 73 U LACTOSA/mg of protein and a yield of 93.3%. Optimun pH and temperature for enzyme activity were pH 6.6 and 40°C. The Km for lactose was 15 mM and V...

In this work we present the design of a Process for the treatment of the urban salid wastes (USW) produced in the northern part of Lima. The plant will be installed in the district of Puente Piedra, region of Lima. The treatment capacily is of 1500 TM/day and ethanol, methane and diesel 2 has been produced of added value, also a higher reduction is achieved to 80% in volume of USW destination at present to the final disposition in the sanitarias fillings fields of Zapallal and The Vizcacha. The impact of the implementation ol this process will be positive so much in the technological, economic and environmental aspects.

Five strains of Kluyveromyces marxianus a one of Gandida pseudotropicalis were cultured in medium M· 1 for productión of β-galactosidase (E.C: 3:2:1:23). The strain Kluyveromyces marxianus NRRL- Y - 1109 was selecteed for the best production (11.6 U/ml). The maximum yield of enzyme (1330 U ONPG/g of biomass) was obtained on 5% of lactose supplemented with 0.5% yeast extract , 0.75% (NH4)2SO4 y 0.45% K2HPO4 (W/V). The best extraction of the enzyme from viable cells was performed by toluene 2% (v/v), at pH 7.0 ± 0.1, 30ºC during 15 hours of treatment, in 0.1 M potassium phosphate buffer, supplemented with 1 mM magnesium and 0.1 mM manganese sulfate. The enzyme was partially purified with acetone. lt obtained a specific activity of de 73 U LACTOSA/mg of protein and a yield of 93.3%. Optimun pH and temperature for enzyme activity were pH 6.6 and 40°C. The Km for lactose was 15 mM and V...

En este trabajo se propone un proceso para el tratamiento de los residuos sólidos urbanos (RSU) generados en el cono norte de Lima. La planta se instalará en el distrito de Puente Piedra, región de Lima. La capacidad de tratamiento es de 1500 TM/dia y se produce etanol, metano y diesel 2 como productos de valor agregado, asimismo se logra una reducción superior al 80% en volumen de RSU destinados actualmente a la disposición final en los rellenos sanitarios de Zapallal y La Vizcacha. El impacto de la implementación de este proceso será positivo tanto en los aspectos tecnológico, económico y ambiental.

It has been developed a process of integration of fermentation systems of organical solid waste (OSW) in facilities of the «La Vizcacha» filling field, Puente Piedra District-Lima. We are obtained 225 mL ethanol and 0,008 5 kWh per kilogramm of urban solid waste.

It has been developed a process of integration of fermentation systems of organical solid waste (OSW) in facilities of the «La Vizcacha» filling field, Puente Piedra District-Lima. We are obtained 225 mL ethanol and 0,008 5 kWh per kilogramm of urban solid waste.