The present work was aimed at determining the antifungal effect in vitro of Apis mellifera L. honey versus environmental fungi (Aspergillus sp., Penicillium sp.) Pathogens and dermatophytes (Epidermophyton floccosum, Microsporum canis and Trichophyton rubrum), cutaneous mycoses are among the significant prevalence of infections in the world, and also determine the chemical composition of honey samples in order to establish ranges of values of some of its main components such as sugars, acidity, moisture and pH indicating its microbial stability. In Chemical Testing was determined pH, acidity, concentration of sugars and humidity values that are within the ranges established. For the antifungal effect in vitro using the disk diffusion method - method of Kirby - Bauer, which made use of two samples of honey from different places of origin, who were identified as (A and B). Was submitted to...