The aim of this work was to synthesize copolymers of styrene (St) and butyl acrylate (AB) cross-linked with allyl methacrylate by semicontinuous heterogeneous polymerization in order to study the mechanical properties of the polymeric films prepared from the latexes obtained. Copolymer latexes were stable and nanoparticles with spherical morphology were observed by Transmission Electron Microscopy (TEM). The latexes had final conversions between 92,5 and 97,0%, which were determined by the gravimetric method, and a solids content close to 20%. The nanoparticles of latex had an average diameter of 59,1; 55,1 and 49,0 nm for monomer dosing rates of 0,75; 0,45 and 0,15 g/min, respectively. By means of mechanical characterization of the polymeric films and according to the statistical analysis, it was found that when the monomer dosage rate decreased in the synthesis, the mean particle diame...

Septal Neuroblastoma (SN 56) cells are hybrid cells made through cell fusions between quiescent medial septum neurons (cholinergic) and tumoral neuroblastoma cells. Cholinergic cells synthesize and release the neurotransmitter acetylcholine. Preliminary studies in our laboratory revealed that SN 56 neurons also express the vesicular glutamate transporter type 1 (VGluT1), a protein that is normally produced by glutamatergic neurons. This discovery prompted us to hypothesize that SN 56 neurons may also co-express a glutamatergic phenotype which is important because glutamatergic neurons have been associated to the pathogenesis of neurological disorders such as Alzheimer’s disease. To assess whether SN 56 neurons express in fact both phenotypes, we conducted experiments in differentiated and no differentiated SN 56 cell, to confirm the expression of glutamatergic phenotype, by qPCR, weste...

Septal Neuroblastoma (SN 56) cells are hybrid cells made through cell fusions between quiescent medial septum neurons (cholinergic) and tumoral neuroblastoma cells. Cholinergic cells synthesize and release the neurotransmitter acetylcholine. Preliminary studies in our laboratory revealed that SN 56 neurons also express the vesicular glutamate transporter type 1 (VGluT1), a protein that is normally produced by glutamatergic neurons. This discovery prompted us to hypothesize that SN 56 neurons may also co-express a glutamatergic phenotype which is important because glutamatergic neurons have been associated to the pathogenesis of neurological disorders such as Alzheimer’s disease. To assess whether SN 56 neurons express in fact both phenotypes, we conducted experiments in differentiated and no differentiated SN 56 cell, to confirm the expression of glutamatergic phenotype, by qPCR, weste...