The author performed serially liver biopsies in adult dogs, using local anesthesia. He used Best´s Carmin for detecting glycogen. In some animals, he investigated the glycemia before each biopsy. He also studied the effect of several substances upon the glycogen content of the liver.

We have described in healthy adult subjects, in infants and in many patients the pathology, an oscillation of the two types of bilirubin in the blood twenty-four hours. This oscillation has periods of accumulation and excretion other, each of them, and is now expected to occur in cycles. We observed that the oscillation is influenced by sleep, food or physical effort. The results of the overcharging test of bilirubin are directly influenced by the cycle time of bilirubinemia; appearing even in normal subjects, inadequate testing, if implemented in periods of accumulation of bilirubin in the blood. Thus, the test of bilirubin loses its significance as an index of liver failure. The study of the various lesions of the liver, and its magnitude, found by the method of biopsies has allowed us to establish that there is no relationship between the anatomical deficiency of the liver and jaundic...

The author discusses the pathogenic causes of certain types of Icterus, making special references to Hemolitic Icterus and Monqe's Disease. In human, as well as in experimental bartonellosis, a great erythrocyte destruction is noticeable, without any increase of blood bilirubin. He has produced massive hemolisis in dogs, and in no case the bilirubin was increased, demonstrating that the normal liver is capable of excreting entirely the bilirubin formed. In Monqe's Disease, the injected bilirubin showed a normal elimination, even though the initial bilirubinemia was above the admitted standard level, just as íf the excretion through the liver cells would occur at a higher threshold. These results are similar to those of Dameshek and Singer in Hemolitic Icterus. The explanation of these phenomena, may be found in a compensatory mechanism which avoid too great a loss of biliary pigments (i...