The aim of this investigation was to study the presence of flaviviruses in rodents from Yucatan, Mexico, by the Polymerase Chain Reaction (PCR) in the semi-nested variant. Ninety rodents were captured (87 Rattus rattus, two Heteromys gaumeri and one Mus musculus), and blood samples were collected. The resulting serum samples were grouped into 30 triads (pools). The semi-nested PCR yielded a positivity frequency of 33.3% (10/30).

The aim of this study was to detect Toxoplasma gondii DNA in skeletal muscle and kidney samples collected from small wild rodents, captured in 10 locations in the Yucatan Peninsula, Mexico (Campeche, Quintana Roo and Yucatan states) in 2018-2019. In total, 127 rodents of seven species were captured. A fragment of skeletal muscle and kidney was taken from all of them for genomic DNA extraction. The identification of T. gondii was carried out by nested PCR directed to a fragment of the B1 gene of the parasite. The rodent species with the highest number of captured individuals was Heteromys gaumeri (n=39). The PCR did not identify T. gondii DNA in the organs evaluated.

The aim of this investigation was to study the presence of flaviviruses in rodents from Yucatan, Mexico, by the Polymerase Chain Reaction (PCR) in the semi-nested variant. Ninety rodents were captured (87 Rattus rattus, two Heteromys gaumeri and one Mus musculus), and blood samples were collected. The resulting serum samples were grouped into 30 triads (pools). The semi-nested PCR yielded a positivity frequency of 33.3% (10/30).

RESUMEN El presente trabajo de investigación tiene por objetivo mejorar la rentabilidad de la Empresa Calzados Paredes SAC. mediante la implementación de un sistema MRP I integrado a las Técnicas de Manufactura Esbelta. Se muestra la situación de dicha empresa y las operaciones asociadas al proceso productivo, luego se establecen puntos a corregir o mejorar para la optimización de los procesos y aumentar la rentabilidad de la empresa. Se inicia la investigación con un marco teórico sobre el sistema MRP y las Técnicas de Manufactura Esbelta a utilizar, que ayudan a entender el contenido del informe, luego se hace una descripción sobre la organización de la empresa, macroprocesos y procesos internos dentro de los cuales nos enfocamos en el proceso productivo. Se establece que las áreas de estudio son almacenes, corte, centrado, habilitado y fierro. El diagnóstico de los problem...