This research aims to describe the cultural elements of a Brazilian public university that can facilitate workplace bullying. It’s characterized as qualitative and descriptive, with the application of an online questionnaire (214 responses), followed by 12 semi-structured interviews. Data was analyzed according to content analysis and descriptive statistics. It was possible to identify cultural characteristics present in both the organizational culture and management of Brazilian public institutions, which, according to participants, favor workplace bullying at the University, such as impunity, power groups, politics, and mischaracterization of public servants. Therefore, the research contributed to the strengthening of the theory, opening opportunities for the development of measures and policies to prevent and combat harassment in universities.