We thank Professor Santiago Mas Coma for suggestions to improve this contribution. This work was supported by grants to JRE from CONCYTEC (Consejo Nacional de Ciencia y Tecnolog?´a-PERU), Proyecto VIGIA-USAID 2003, The International Foundation for Science contract B/2856-1, Sweden and INCAGRO-PERU /Contract 007-2003.

Fascioliasis is an infectious parasitic disease distributed globally and caused by the liver fluke Fasciola hepatica or F. gigantica. This neglected tropical disease affects both animals and humans, and it represents a latent public health problem due to the significant economic losses related to its effects on animal husbandry. For decades, triclabendazole has been the unique anti-Fasciola drug that can effectively treat this disease. However, triclabendazole resistance in fascioliasis has more recently been reported around the world, and thus, the discovery of novel drugs is an urgent need. The aim of this study was to investigate the fasciocidal properties of 400 compounds contained in the Pathogen Box. The first stage of the screening was carried out by measuring the fasciocidal activity on metacercariae at a concentration of 33 M each compound (the standard dose). Subsequently, the ...