Spirulina is a multicellular, photosynthesis, filamentous blue-green algae which is found naturally in a wide range of fresh, marine, and brackish waters. It is an excellent source of protein, vitamins, minerals, lipids, carbohydrates, and pigments thus considered as the “superfood” of the century. The commercial production of Spirulina depends on many factors such as nutrient availability, temperature, and light. Zarrouk’s medium is known to be the standard media (SM) which contains all the macro and micro-nutrients required for the growth of Spirulina. The cost of nutrients is however found to be the second limiting factor next to labor cost affecting the commercial-scale production of Spirulina. The cost of carbon source is higher than that of N and P sources. Therefore, this study aimed at developing a low-cost medium for large-scale production of Spirulina. This intention was ...