Background: Arboviral diseases has reemerged in the last years affecting primarily the coast of Peru. Piura is an endemic region of Dengue, where the recent appearance of Chikungunya (CHIKV) and Zika (ZIKV) has been renewed. Aim: To assess the presence of DENV, CHIKV, and ZIKV in serum samples of patients with acute febrile illness in a locality of Piura, Peru and describe associated clinical features. Methodology: Descriptive cross-sectional study, sampling was done for convenience where it was obtained a total of 496 serum samples from patients with acute febrile illness were collected from 18 primary care centers. All samples were analyzed via real-time RT-PCR to detect DENV, CHIKV, and ZIKV. In addition, positive samples for DENV were also processed for serotypes 1 to 4 classification via RT-PCR. Results: DENV was the most common arbovirus detected in 170/496 (34.3%), followed by ZIK...