Dicistroviruses are a family of viruses that affect invertebrates of sanitary and economic importance. Pigeons are also natural reservoirs of pathogens that have caused emerging and re-emerging diseases in humans. Twenty-five pigeons (24 Columba livia and one Zenaida auriculata) were captured in La Plata, Buenos Aires, between May and June 2019. Oropharyngeal/choana and then cloacal swabs were taken from each bird. RNA extraction was performed with Trizol®. For the analysis, 5 μl of total RNA was used for complementary DNA (cDNA) synthesis. The reaction was carried out using multiplex PCR (mPCR) as screening methodology in a final volume of 25 μl. This multiplex PCR amplifies six viruses. As a positive control, a sample previously isolated and characterized in the authors' laboratory as Israeli acute paralysis virus (IAPV) was used, and whose presence was confirmed by screening using ...