Neonatal domestic South American Camelid llamas and alpacas suffer from an enteric disease complex characterized by abdominal distention, lethargy, dehydration, and eventual fatal septicemia. Analysis of rectal swabs from neonatal alpacas suffering clinical diarrheas has constantly isolatedEscherichia coli, mainly the EPEC and EHEC pathotypes. The present communication reports the results of molecular analysis of 226E. colistrains from neonatal alpaca rectal swabs. The isolates were initially tested by multiple PCR, to identifyE. colivirulence geneseae,bfp,Lt,Stx1,Stx2,sta,stab, andltgenes and a similar test to detect F4, F5, F6, F17, and F41fimbriae adhesin genes. Forty-two of the 226 (18.5%) isolates tested positive for at least one pathogenic gene, 25 of the 42 were classified as EPEC (3 positives for onlyeaeand 22 for botheaeandbfp) and the remaining 17 were classified as EHEC. Twent...

El objetivo del presente estudio fue identificar y evaluar la susceptibilidad antibiótica de los principales patógenos bacterianos aislados de casos de linfadenitis cervical en cuyes procedentes de dos centros de crianza familiar-comercial de la provincia de Canchis, Cusco. Se procesaron 64 muestras de abscesos cervicales entre setiembre de 2018 y marzo de 2019. Las muestras fueron cultivadas y se procedió con el antibiograma en agar con discos de los principales antibióticos usados en sistemas de crianza familiar-comercial. Se identificó a Streptococcus sp beta-hemolítico (91.8%, 56/61), Staphylococcus sp (45.9%, 28/61) y Klebsiella sp (21.3%, 13/61). La mayor frecuencia de aislados se presentó en cuyes adultos. La frecuencia de susceptibilidad antimicrobiana resultó alta para todos los géneros aislados, a excepción de la enrofloxacina y trimetoprim-sulfametoxazol para Strepto...

The aim of this study was to identify and evaluate the antibiotic susceptibility of the main bacterial pathogens isolated from cases of cervical lymphadenitis in guinea pigs from two commercial-family breeding centres in the province of Canchis, Cusco. In total, 64 samples of cervical abscesses were processed between September 2018 and March 2019. The samples were cultured and the antibiogram on agar with discs of the main antibiotics used in family-commercial rearing systems was carried out. Beta-hemolytic Streptococcus sp (91.8%, 56/61), Staphylococcus sp (45.9%, 28/61) and Klebsiella sp (21.3%, 13/61) were identified. The highest frequency of isolates occurred in adult guinea pigs. The frequency of antimicrobial susceptibility was high for all the isolated genera, except for enrofloxacin and trimethoprim-sulfamethoxazole for beta-hemolytic Streptococcus sp. The results show a diversit...

En el Perú se vienen reportando serovares de Salmonella no tifoidea (SNT) en casos humanos que estarían relacionados a los hallados en huevos comerciales, carcasas de broilers y subproductos. El presente estudio tuvo como objetivo determinar mediante el porcentaje de similaridad de los perfiles genéticos de los serovares de SNT aislados de humanos y productos aviares mediante la Electroforesis en Gel de Campo Pulsado (PFGE). Se obtuvieron 49 aislados bacterianos de serovares de SNT de casos clínicos humanos y productos aviares (huevos, carcasa de broilers, vísceras, cama de ave), que se analizaron mediante PFGE. Con el programa bioinformático Bionumerics v. 7.0 se generó un dendrograma que muestra la relación XbaI – perfiles PFGE. El análisis de las bandas generadas se realizó mediante el uso del Método de grupo de pares no ponderados con media aritmética (UPGM) para evalua...

The study aimed to describe the microbiota present in alpaca semen. Fifteen semen samples were collected using the artificial vagina, electroejaculation and post-copula techniques (five per technique). In addition, preputial (n=5) and vaginal (n=5) swabs were made. Microbiological cultures were made to determine the bacterial and fungal genera and an assessment of the bacterial load by determining the number of colony-forming units (CFU/ml). Six bacterial genera were identified in the vagina and seven in the prepuce, in addition to a fungal species (Aspergillus fumigatus). Bacteria of the genera Escherichia, Staphylococcus, Streptococcus and Bacillus were the most abundant and present in semen obtained by the three techniques, coinciding with those found in preputial and vaginal swabs. The amount of CFU/ml was similar for the three techniques, while there was no presence of fungi in the ...