Water limitation is one of the most serious problems in tomato production, responsible for a significant reduction in productivity and fruit quality. In this context, the application of exogenous proline may be an alternative for plants to deal with possible water stress. The objective of this work was to evaluate the production, dry matter mass partition and organic carbon content of the tomato cultivar “Vivacy”, cultivated with doses of proline and irrigated every seven days. A randomized block design was used, with four replications and a 2 x 3 + 1 factorial arrangement. The factors consisted of 2 doses of proline (100 and 150 mg L-1), 3 application times (1, 3 and 6 days after irrigation) and a control without proline application. Data analysis showed a significant difference in the variables analyzed, revealing that the application of proline influenced the production and partit...