The reproductive activity of various species of mammals is influenced by the sperm subpopulations of their ejaculates. These subpopulations differ in the motility and kinetic characteristics of the sperm and have been related and classified according to their capacity for the fertilization process. The objective of this research was to evaluate the effect of the composition of the semen diluent on the sperm subpopulations of the chilled rabbit semen. The semen of 10 rabbits of the Californian breed (three ejaculated per animal) was obtained. Each ejaculate was divided into four aliquots, which were diluted in one of four diluents, whose basic composition consisted of T1: skim milk and sugars; T2: dextrose, sodium citrate and potassium acetate; T3: sodium caseinates, phosphates and sugars; and T4: tris-citric acid and egg yolk. Each aliquot was kept refrigerated at 16 °C for 72 h. I...

The aim of this research was to evaluate the use of different extenders for cooling of rabbit semen and its effect on sperm quality. Thirty semen samples from 10 sexually mature rabbits (Oryctolagus cuniculus) were used. Each sample was diluted separately in a 1:10 ratio in four extenders, composed of skim milk and sugars (T1); dextrose, sodium citrate, and potassium acetate (T2); sodium caseinates, phosphates and sugars (T3); and tris-citric acid and egg yolk (T4). The semen was kept at 16 °C for 72 hours. At the time of dilution and every 24 hours, total motility (MT), progressive motility (MP) and sperm kinetics were evaluated with the Sperm Class Analyzer® system, and vitality and morphology were evaluated by the eosin-nigrosin staining. Statistical analysis was performed using mixed models and the comparison of means by the Duncan test. At the time of dilution (0 h), a lower MT fo...

The aim of this research was to evaluate the use of different extenders for cooling of rabbit semen and its effect on sperm quality. Thirty semen samples from 10 sexually mature rabbits (Oryctolagus cuniculus) were used. Each sample was diluted separately in a 1:10 ratio in four extenders, composed of skim milk and sugars (T1); dextrose, sodium citrate, and potassium acetate (T2); sodium caseinates, phosphates and sugars (T3); and tris-citric acid and egg yolk (T4). The semen was kept at 16 °C for 72 hours. At the time of dilution and every 24 hours, total motility (MT), progressive motility (MP) and sperm kinetics were evaluated with the Sperm Class Analyzer® system, and vitality and morphology were evaluated by the eosin-nigrosin staining. Statistical analysis was performed using mixed models and the comparison of means by the Duncan test. At the time of dilution (0 h), a lower MT fo...