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artículo
Objective. To determine the antimicrobial effect of ethanolic extracts from Caesalpinia spinosa against penicillin and methicillin resistant strains of Staphylococcus aureus penicillin and methicillin. Methods. S. aureus ATCC 11632 and 33592 strains were used, ethanolic extracts from C. spinosa leaves and pods were obtained by maceration at concentrations of 25%, 50%, 75% and 100%. The Kirby-Bauer method was used, where filter paper discs were loaded with leaf, pod and seed extracts and deposited on the medium, inoculated with a 0.5 McFarland suspension of S. aureus. Ampicillin discs were used as positive control and ethanol-impregnated discs as negative control. After 24 hours, the diameters of the halos were measured with a Vernier caliper. Results. Haloes up to 18 mm in diameter with 100% leaf extracts, 17 mm with pod extracts and 14 mm with seed extracts were recorded against S. aur...
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artículo
Objective: To assess the presence of microorganisms on the surfaces of sterile instruments in the periodontics area of a university pre-professional practice clinic. Materials and methods: A descriptive and observational study, in which 100 randomly selected samples of periodontics instruments were selected. The samples collected were analyzed using cultures on specific media, including blood agar, Sabouraud agar, and eosin methylene blue agar, which enabled the primary identification of four classes of microorganisms: Gram-positive cocci, Gram-positive bacilli, Gram-negative bacilli, and yeasts. Results: Of the instruments analyzed, 19% (n = 19) were contaminated. The highest contamination rate was observed in Gracey 3/4 periodontal curettes, with 30% (n = 12), in which only gram-positive cocci were identified, with no positive results for gram-positive bacilli, gram-negative bacilli, o...