Has immobilized the snake venom of Bothrops atrox Peruvian to obtaining specific antibodies from the serum of immunized previously albino rabbits by affinity chromatography . Venom ( 100 mg ) was coupled to 1 g of BrCN - Sepharose 4B resin and packed into a chromatography column of 0.8 x 6.0 cm . This column was used for purification of specific antibodies from serum of New Zealand albino male rabbits ( 2 kg ) immunized with B. atrox venom . Serum ( 1 ml ) was applied to the column and protein fractions were eluted sequentially with 0.15 M NaCl , pH 6.0 buffer and 0.1 M glycine -HCl , pH 2.5 . Protein quantitation was performed by spectrophotometric methods . Immobilization of B. atrox venom gel yielded the affinity column to obtain anti- B antibodies . atrox , which were eluted with glycine -HCl buffer 0.1 M , pH 2.5 , recovering 100 mg of this antibody per mL serum. It is concluded tha...

Has immobilized the snake venom of Bothrops atrox Peruvian to obtaining specific antibodies from the serum of immunized previously albino rabbits by affinity chromatography . Venom ( 100 mg ) was coupled to 1 g of BrCN - Sepharose 4B resin and packed into a chromatography column of 0.8 x 6.0 cm . This column was used for purification of specific antibodies from serum of New Zealand albino male rabbits ( 2 kg ) immunized with B. atrox venom . Serum ( 1 ml ) was applied to the column and protein fractions were eluted sequentially with 0.15 M NaCl , pH 6.0 buffer and 0.1 M glycine -HCl , pH 2.5 . Protein quantitation was performed by spectrophotometric methods . Immobilization of B. atrox venom gel yielded the affinity column to obtain anti- B antibodies . atrox , which were eluted with glycine -HCl buffer 0.1 M , pH 2.5 , recovering 100 mg of this antibody per mL serum. It is concluded tha...