The purpose of the study was to achieve the isolation and purification of Vitexin from Vitex pseudolea Rusby, which is the active principal present in several species of the genus Vitex due to its phylogenetic properties that characterize it as belonging to a monophyletic family. The sample was collected in the town of Tamshiyacu, District of Sgto. Fernando Lores Tenazoa, Maynas, Loreto, the heartwood was taken to sawdust for extraction with solvents. The active principle was characterized by determining its physicochemical properties and by UV-Visible spectrometry. 2.352 g of vitexin were obtained, the melting point was 204 ° C., Soluble in methanol, ethanol and hot water. The colored tests were positive for flavonoid, the molecular weight calculated by the Rast method gave 432.37 g / mol ± 0.049. The Rf values were made with various solvent mixtures, using a mixture of ethyl acetate:...