The present investigation had the purpose of: determining the differences in the in vitro development of buds from croton young plants of latex producers; to evaluate the response to the acclimatization of croton’s plantulas with better behavior in vitro, and to determine the chromosomal number of selected individuals of croton latex producers. The culture medium for the in vitro introduction was determined evaluating the behavior of croton draconoides in five treatments with the basic medium murashige and skoog (ms) taking into consideration that the species in the study belong to the same gender. The buds coming from croton young plants producers of wine latex had the best behavior in the selected culture medium (ms + 0,01mg/i ana + 0,1mg/i of baP and aG3 + 20 ml of coconut water) presenting an increase in height, number of leaves and percentage of survival in relationship to croton ...