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1
tesis de maestría
La Tripanosomiasis americana es aún un problema de salud pública en la región Arequipa, por tal razón se realizó un estudio en escolares de la IE San José de Tiabaya; con el objetivo de determinar la seroprevalencia, las características electrocardiográficas (EKG) y ecocardiográficas (ECCG), más frecuentes de los casos seropositivos, así como describir las características epidemiológicas y los conocimientos sobre Triatoma infestans (“Chirimacha”). Para ello se estudiaron 381 escolares, con consentimiento informado, mediante los métodos de ELISA e IFI y se realizaron EKG y ECCG en el HRGHD, y se aplicó una ficha epidemiológica. La seroprevalencia a Trypanosoma cruzi en los escolares fue de 7.09% (27/381), la característica más frecuente de los casos seropositivos a T. cruzi, al EKG y al ECCG es normal con 88.9% y solo el 11.1% presento alteraciones cardiacas, un caso...
2
artículo
Objective: To determine the value of indirect immunofluorescence (IFI) in the diagnosis of leishmaniasis and Chagas disease. Material and method: Indirect immunofluorescence (IFI) was employed for diagnosing leishmaniasis and Chagas disease using Trypanosoma cruzi epimastigota as substrate antigens. A nuclear fluorescence pattern was found in sera from patients with leishmaniasis, and a peripheral pattern was found in those with Chagas disease. Afterwards, we estimated the diagnostic value of IFI for both diseases in a blinded fashion. Results: For diagnosing leishmaniasis, IFI sensitivity was 84.2%, specificity, 100%, positive predictive value, 100%, negative predictive value 82.9%, and accuracy was 91.0%. Conclusions: The immunofluorescence technique using Trypanosoma cruzi epimastigota, considering the proposed color patterns, is useful for diagnosing cutaneous lei...
3
artículo
Objective: To determine the value of indirect immunofluorescence (IFI) in the diagnosis of leishmaniasis and Chagas disease. Material and method: Indirect immunofluorescence (IFI) was employed for diagnosing leishmaniasis and Chagas disease using Trypanosoma cruzi epimastigota as substrate antigens. A nuclear fluorescence pattern was found in sera from patients with leishmaniasis, and a peripheral pattern was found in those with Chagas disease. Afterwards, we estimated the diagnostic value of IFI for both diseases in a blinded fashion. Results: For diagnosing leishmaniasis, IFI sensitivity was 84.2%, specificity, 100%, positive predictive value, 100%, negative predictive value 82.9%, and accuracy was 91.0%. Conclusions: The immunofluorescence technique using Trypanosoma cruzi epimastigota, considering the proposed color patterns, is useful for diagnosing cutaneous lei...