In the present investigation, the physicochemical characteristics, composition of fatty acids, tocopherols and phytosterols of the oil obtained from granadilla and pumpkin seeds were determined using supercritical CO2 technology. The fruits of granadilla (Passiflora ligularis Juss) and pumpkin (Cucurbita maxima) were obtained and the seeds were extracted, then dehydrated at 60 ° C x 24 hours and subsequent grinding to obtain the oil in a supercritical fluid extractor under the conditions of 350 bar and 50 ° C and CO2 flow of 3mL / min. The composition of fatty acids were analyzed, in the saponifiable fraction of the oils, arachidonic and oleic acid and 82.37 % of unsaturated fatty acids in granadilla seed and oleic, Arachidonic and linoleic acid and 76.01 % of fatty acids were found unsaturated in pumpkin seed and in the unsaponifiable fraction presented α-Tocopherol of 17.85 μg / g ...